We performed an experiment to find bacteriophage growth/ burst size. Nobody got good results that make sense, and our teacher asked us this. "The experiment we performed to determine the average number of mature virions released did not work. We tried to syringe filter the virus before we infected the bacteria and then plated. Think about how we performed this experiment and offer an explanation as to why the experiment may not have worked."
I don't understand what went wrong. I'm sure it has something to do with syringe filtering the virus because she mentioned it in the question? Should we not have syringe filtered the virus for some reason? Please help me understand!
Unforturnetly, I don't know the sizes of the filter pores.
Syringe filters are used to remove cell debris, bacteria, mycoplasma from any biological media. Mostly syringe filters of 0.45 microns shall be used to remove these organisms and debris. If 0.2 microns are used, it will reduce the titer value of virus. Hence it cannot infect bacteria effectively. In ur case you should have used smaller size pores which reduced the concentration of virus.
We performed an experiment to find bacteriophage growth/ burst size. Nobody got good results that make...