Question

Chapter 3 Home Active and Due Dates Chapter Homework Resouros Ghve Up! Feedback PAGE A protein sample complex consists of two proteins, a smaller protein, X, and a larger protein, Y. Protein X is composed two polypeptide chains linked by disulfide bonds. Protein Y is composed of three polypeptide chain linked by disulfide bonds The complex is analyzed by native PAGE, reducing SDS-PAGE and non-reducing SDS-PAGE Native PAGE does not include sodium dodecyl sulfate, or SDS. Reducing SDS-PAGE uses both SDS and a reducing agent in the buffer Non-reducing SDS-PAGE uses SDS, but no reducing agent. Match each gel pattern to an experimental method. The first line in cach gel is a molecular weight ladder Native PAGE Non-reducing SDS-PAGE Reducing SDS PAGE 1000D IT III What are the molecular weights of the protein X polypeptides! 60 LD and 30 kDa What are the molecular weights of the protein Y polypeptides! GOD, SokDa, and 30 Da PrtScn POUP Pgo End Home

Answer 1

Protein x is smaller hence the smaller size observed in reducing SDS page is 100 kDa and it is composed 2 polyptides 50 kDa and 50 kDa bound by disulfide bond

Protein y is larger hence the larger size observed in resucing SDS page is 150 kDa and it is composed of 3 polypetides of 60 kDa and 60 kDa and 30 kDa size