In the protocol we followed, 16S rRNA gene segments were amplified by PCR, then individually ligated into a vector, transformed and propagated in bacteria, and 9 bacterial “clones” sequenced by the Sanger method. Each clone had a unique sequence we subsequently compared. If we had used an Illumina Next Generation Sequencing method, would the “cloning” of individual fragments have been necessary?
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In the protocol we followed, 16S rRNA gene segments were amplified by PCR, then individually ligated...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...