Question

8. (8) pDXA-GFP2 is the base vector for pCT-GFP. What elements for this plasmid have been important to the labs that we have completed in the first half of the semester? List the element and then describe in one or two sentences what role it played in our experiment. I anticipate that a complete answer will list four elements. (Write answer on back) Xhol Xba 1 BamHI Sac .- EcoRI- Kpn 1 EcoR V- Hind Ill- Sall Sphi G418 Ddp2 PDXA-GFP2 6.87 kb Cla! - EcoR v Amp

Answer 1

Answer 8)

There are found following elements in the vector:

* GFP: It stands for green fluorescent protein. This is used to detect the position of plasmid in the cell. Because there is found green fluoresecence because of this gene expression.

* Amp: These are the genes which provides the ampicillin resistance to the bacteria. This allow bacteria to grow even in presence of ampicillin.

*G418: It provides the resistance to bacteria against genticin. When this gene is not found then there is not found elongation step in protein synthesis.

*Ddp2: It stands for DNA distortion protein. 2. It maintains the structure of the DNA.

Other gene essential for plasmid:

*ori: It stands for origin of replication. This allow the replication process in the bacteria.

* Restriction sites: This are cleaved by endonuclease and allow the insertion of gene of interest in the plasmid.