Suddenly put E. coli in 20% NaCl solution and distilled water respectively. What will happen? If...
Suddenly put E. coli in 20% NaCl solution and distilled water
respectively.
What will happen?
If various nutrients for growth were added to the the above solvent, in which case would the growth of E. coli be supported?
What's the reason?
Homework Answers
E. coli cells in 20% NaCl solution provides hypertonic
environment. Thus, E. coli cells lose water to the environment as
there is movement of water out of the cell to an area of higher
solute concentration which means to 20% NaCl solution.
But in distilled water that gives hypotonic environment. There is
movement of water into the cell cytoplasm and it may cause
lysis.
If growth nutrients added to the distilled water that bring the
solution to an isotonic state. Then growth of E. coli will be
supported.
But, growth of E. coli will not supported if growth nutrients added
to 20% NaCl solution as the cells may be halophilic which means may
adapted to highly saline conditions for their survival in such an
environment.
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Suddenly put E. coli in 20% NaCl solution and distilled water
respectively.
What will happen?
If...
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If you take a 171 mL of a 1.4 M NaCl solution and dilute it to
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Enter the numerical answer in decimal notation
QUESTION 10
Information - Colligative
Properties
Colligative properties of solutions are those properties which
depend only on the number of dissolved solute particles in solution
not the chemical properties of the solute. For example when a
solute is dissolved in a solvent, vapor pressure
depression...
1. Fill in the table above with what you observe on your
plates.
2. Bacterial transformation occurred on which agar plate (s)?
What evidence do you have that the bacteria were transformed
here?
3. Which plates have glowing growth? Explain what causes
bacteria to glow.
II. Transformation of E. coli with Plasmid DNA (PGLO) 1. Three LB (Luria Broth) agar plates are obtained. The plates contain: • Plate A: LB-Agar/Ampicillin/Arabinose • Plate B: LB-Agar/Ampicillin • Plate C: LB-Agar 2. Three...
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