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IV. EXERCISE 3. DESIGNING AN EXPERIMENT. In this exercise you will work on the same reaction...
3) You can follow the above reaction in a test tube using a dye that turns purple in the presence of the product (the more product that is made, the more purple the solution). You discover an inh...
3) You can follow the above reaction in a test tube using a dye that turns purple in the presence of the product (the more product that is made, the more purple the solution). You discover an inhibitor of Enzyme A and want to determine if it is competitive or non-competitive inhibitor. You set up the following reactions and measure the amount of purple using a spectrophotometer. Results are below: Absorbance Conditions Test tube 0.63 Enzyme + substrates Inhibitor added...
In order to measure the rate of this reaction, we utilized the fact that starch -...
In order to measure the rate of this reaction, we utilized the fact that starch - in the presence of iodine has a distinct dark blue color while the conversion of starch to its monomer leads to a more transparent/amber colored solution. While you used a simple visual analysis to verify the conversion, it is also possible to quantify the change in (starch) using a spectrophotometer. 1. Why/how was a spectrophotometer used for this purpose? EXPLAIN what this machine does...
Question Completion Status: THE TOWN CAP WIVE cuvy UE CE POTURU U UR ECCE v CK...
Question Completion Status: THE TOWN CAP WIVE cuvy UE CE POTURU U UR ECCE v CK CONTENIX catalyzes the breakdown of hydrogen peroxide with the production of water and oxygen. Certain dyes will go from a colorless to a colored form upon oxidation, a property that allows detection of peroxidase activity. In the presence of hydrogen peroxide and peroxidase, orthophenylenediamine (OPD) w be changed from a colorless dye to a yellow compound as it is oxidized. peroxidase H2O + OPD...
Your lab instructor has given you a protocol to perform a molecular cloning experiment. In a...
Your lab instructor has given you a protocol to perform a molecular cloning experiment. In a previous experiment, you used polymerase chain reaction (PCR) to amplify a sequence that you believe to regulate expression of a gene you are studying. You will now take this purified PCR product (double stranded DNA) and ligate it into a plasmid that contains a luciferase reporter gene. If your DNA sequence is a promoter sequence, then its presence will allow for expression of the...
please answer them all. When designing a sound scientific experiment, which of the following guidelines should...
please answer them all.
When designing a sound scientific experiment, which of the following guidelines should be followed? Isolating one independent variable makes it simpler to evaluate for a causal relationship. Correlational data can be used to prove a hypothesis. The control group is ignored during comparison. Constants are not always necessary. The process known as gastrulation results in the formation of which of the following embryonic structures? . . . . . Coelomic cavities Germinal layers Circulatory systems Neural...
In this experiment, you will measure the rate of photosynthesis in isolated chloroplasts using the dye...
In this experiment, you will measure the rate of photosynthesis
in isolated chloroplasts using the dye 2,6-dichloroindophenol
(DPIP). The transfer of electrons during the light-dependent
reaction results in the production of H+. This H+ ion can reduce
DPIP, changing its color from blue to clear. The color can be
quantitatively measured with a spectrophotometer. However, in this
experiment, you will qualitatively analyze your data.
Write a detailed conclusion for your experiment. How did light
affect the time it took for...
Research is an integral part of all professional fields. Designing a research study can be a...
Research is an integral part of all professional fields. Designing a research study can be a complicated task that can be simplified if the appropriate techniques can be identified. This assignment will give you the opportunity to design an experiment, including finding relevant prior research, determining the appropriate sample, data analysis techniques, and discuss the results you hope to see. Working from the topic chosen earlier in the topic selection, you will be designing your own statistical study. In a...
Homework for lab 4: Complete exercise 4 page 76-77-78 (question 6 only for page 78). Design...
Homework for lab 4: Complete exercise 4 page 76-77-78 (question 6 only for page 78). Design your own experiment to examine the effect of pH on reaction rate. Material: • Solution of pH 5, pH 7 and pH 9 • Catechol • Potato extract (that's the enzyme) Water (optional) . . . Answer those questions (p77-78) what is your hypothesis What are your dependant and independant variables What are your controls • Briefly explain your experiment below • Design a...
EXERCISE 5B CELLULAR RESPIRATION Alcoholic Fermentation by Yeast OBJECTIVES the completion of thi...
EXERCISE 5B CELLULAR RESPIRATION Alcoholic Fermentation by Yeast OBJECTIVES the completion of this exercise the student should be able to Upon 1 distinguish between the dependent and independent variables in a controlled experiment. . formulate a basic hypothesis. measure the volume of carbon dioxide generated by anacrobic fermentation in baker's 3. yeast. 4. express data in tabular form. 5, relate data generated in an experiment to a hypothesis previously formulated 6. graph data, calculate the slope of a line, and...
Question 3: In a lab experiment, you are tasked with determining the electrochemical reaction that can...
Question 3: In a lab experiment, you are tasked with determining the electrochemical reaction that can occur between aqueous hydrochloric acid (HCl)) and solid magnesium metal (Mg). When the magne- sium is placed in solution, gas bubbles begin to appear around the metal and evolve from solution. (a) Write out the two half reactions that occur when the magnesium is placed in the hydrochloric acid solution. Which species is the getting oxidized? Reduced? (b) Which species is acting as the...
3) You can follow the above reaction in a test tube using a dye that turns purple in the presence of the product (the more product that is made, the more purple the solution). You discover an inhibitor of Enzyme A and want to determine if it is competitive or non-competitive inhibitor. You set up the following reactions and measure the amount of purple using a spectrophotometer. Results are below: Absorbance Conditions Test tube 0.63 Enzyme + substrates Inhibitor added...
In order to measure the rate of this reaction, we utilized the fact that starch - in the presence of iodine has a distinct dark blue color while the conversion of starch to its monomer leads to a more transparent/amber colored solution. While you used a simple visual analysis to verify the conversion, it is also possible to quantify the change in (starch) using a spectrophotometer. 1. Why/how was a spectrophotometer used for this purpose? EXPLAIN what this machine does...
Question Completion Status: THE TOWN CAP WIVE cuvy UE CE POTURU U UR ECCE v CK CONTENIX catalyzes the breakdown of hydrogen peroxide with the production of water and oxygen. Certain dyes will go from a colorless to a colored form upon oxidation, a property that allows detection of peroxidase activity. In the presence of hydrogen peroxide and peroxidase, orthophenylenediamine (OPD) w be changed from a colorless dye to a yellow compound as it is oxidized. peroxidase H2O + OPD...
please answer them all.
When designing a sound scientific experiment, which of the following guidelines should be followed? Isolating one independent variable makes it simpler to evaluate for a causal relationship. Correlational data can be used to prove a hypothesis. The control group is ignored during comparison. Constants are not always necessary. The process known as gastrulation results in the formation of which of the following embryonic structures? . . . . . Coelomic cavities Germinal layers Circulatory systems Neural...
In this experiment, you will measure the rate of photosynthesis
in isolated chloroplasts using the dye 2,6-dichloroindophenol
(DPIP). The transfer of electrons during the light-dependent
reaction results in the production of H+. This H+ ion can reduce
DPIP, changing its color from blue to clear. The color can be
quantitatively measured with a spectrophotometer. However, in this
experiment, you will qualitatively analyze your data.
Write a detailed conclusion for your experiment. How did light
affect the time it took for...
Homework for lab 4: Complete exercise 4 page 76-77-78 (question 6 only for page 78). Design your own experiment to examine the effect of pH on reaction rate. Material: • Solution of pH 5, pH 7 and pH 9 • Catechol • Potato extract (that's the enzyme) Water (optional) . . . Answer those questions (p77-78) what is your hypothesis What are your dependant and independant variables What are your controls • Briefly explain your experiment below • Design a...
EXERCISE 5B CELLULAR RESPIRATION Alcoholic Fermentation by Yeast OBJECTIVES the completion of this exercise the student should be able to Upon 1 distinguish between the dependent and independent variables in a controlled experiment. . formulate a basic hypothesis. measure the volume of carbon dioxide generated by anacrobic fermentation in baker's 3. yeast. 4. express data in tabular form. 5, relate data generated in an experiment to a hypothesis previously formulated 6. graph data, calculate the slope of a line, and...
Question 3: In a lab experiment, you are tasked with determining the electrochemical reaction that can occur between aqueous hydrochloric acid (HCl)) and solid magnesium metal (Mg). When the magne- sium is placed in solution, gas bubbles begin to appear around the metal and evolve from solution. (a) Write out the two half reactions that occur when the magnesium is placed in the hydrochloric acid solution. Which species is the getting oxidized? Reduced? (b) Which species is acting as the...
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