1. To detect genome wide variation aCGH, SNP array, exome/genome sequencing, karyotype are used.
aCGH is Comparative genomic hybridization helps in the detection of variation in copy no.
SNP array is single nucleotide polymorphism is type of DNA microarray used to detect genomic variation across population
exome/genome sequencing is the sequencing of all protein coding genes of the DNA.
karyotype is the pairing and ordering of all the chromosomes of a organism thus providing a picture of the complete chromosome of an individual.
2. Techniques to detect copy no. variation are aCGH, Southern blot analysis, quantitative PCR, FISH and karyotype
aCGH is mostly used to detect copy no. variation.
Southern blot analysis detection by DNA hybridization analysis
quantitative PCR will detect by amplifying the DNA. If the coly no. is more, no. of copies will be more.
Which of the following techniques detect genome-wide variation? Allele specific PCR and capillary electrophoresis, MLPA O...
and w Two-dimensional gel electrophoresis separates proteins based on a. shape; charge Ob.size; concentration c. concentration; shape O d. size, charge O e. size; shape Refer to the table. Several strains of a bacterium are sequenced to investigate the pan and core genomes. In the table, + denotes presence of the gene and denotes its absence. Gene Gene Gene Gene Gene Strain ! Strain 2 + Strain 3 + Strain 4 + + + + Strain 5 + + What...