When setting up the polymerase chain reaction, approximately 200 ng of DNA should be added in...
When setting up the polymerase chain reaction, approximately 200 ng of DNA should be added in a 50 ul reaction. How many ul of the above (it was 115 ng/ul) undiluted DNA solution should be added to the PCR tube
Homework Answers
We need 200 ng DNA in 50 ul of reaction mixture.
115 ng DNA = 1 ul
1 ng DNA = 1/115 ul
200 ng DNA = 1/115 × 200 = 1.739 ul
We need to add 1.739 ul of 115 ng/ul to the reaction mixture.
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