Question

1. If you were trying to come up with an easy-to-remember analogy for the polymerase chain reaction, you would likely say PCR is similar to a colored pencils scissors dishwasher photocopier 2. What determines the piece of DNA that is amplified in PCR? The specific primers used The source of the DNA The temperature of each cycle The specific restriction enzyme used 3. What is the role of temperature in PCR? It is used to separate and anneal the nucleic acids It is required for the use of restriction enzymes

Answer 1

que 1 if u were trying to come up with an easy to remember for the PCR, you would like to say PCR is similar to

• Photocopier

By using photocopier Machine you can make as many copies as you want but this machine will only copy the selected region of the data to copied.

So the PCR works same , make many copies of specific DNA region.

que what determines the pieces of DNA that is amplified by PCR?

• the specific primeres used

Primer is used to amplify the DNA for the production of multiple copies of DNA in PCR.

Primer design specific regions of DNA to amplified in many copies of DNA by PCR.

Que what is the role of temperature in PCR?

• It is used to separate and anneal the nucleic acid

During the temperature steps in PCR: In denaturation steps heat the sample between 94° - 98° C to cause denaturation of template DNA strands , and after the strands are seperate , the temperature is decreased to annealing temperature to allow the primer to anneal of complementary region of template DNA.

Que what best explain how PCR can be used to detect an infection.

• specific primeres are used that would result in the amplification of DNA from an infectious agents if it was present in the patient thus amplification can be seen with the presence of specific size fregment when PCR products is run on a gel.

By using PCR , infectious DNA sample is amplified through PCR by using specific primer. Which bind with that infectious target site of DNA , and producing enough copies of DNA to analyse.

For visualisation of PCR reaction using gel electrophoresis. In which DNA are run through gel matrix by electric current, after that it seperate according to a size of DNA. Also a standard DNA run on ladders having size of the fragments in PCR sample can be determined. And a DNA band can be visualised.