Question

3. A simple batch fermentation of an aerobic bacterium growing on methanol gave the results shown in the following table.  

Time (h)	X (g/L)	S (g/L)
0	0.2	9.23
2	0.211	9.21
4	0.305	9.07
8	0.98	8.03
10	1.77	6.8
12	3.2	4.6
14	5.6	0.92
16	6.15	0.077
18	6.2	0

1. Plot ln(X) vs time and label each phase of growth
2. Calculate the following…

1. Specific growth rate
2. Doubling time
3. Yield coefficient, Yx/s

Answer 1

A X2x1et B Douby Trme ta- 2 td=2.3) ©१४ 0.54 A(2) A o.3 Yiad Coebs cet x/s p629/2 So 9.235L Spo 9)L 29/L -Xo -2-02 -0 65 So- Sp 9.23 0

2 Stationary 1,5 1 0.5 Log 0 Series1 5 10 15 20 -0.5 -1 1.5 Lag -2 Time 9 LC х ит