The UV absorbance of a solution of double-stranded DNA is monitored at 260 nm as a function of Temperature. From the data collected, determine the melting temperature of the DNA. Paste the plot you used to make this decision below.
T(K) |
343 |
348 |
353 |
355 |
357 |
359 |
361 |
365 |
370 |
Absorbance |
0.30 |
0.35 |
0.50 |
0.75 |
1.22 |
1.40 |
1.43 |
1.45 |
1.47 |
The UV absorbance of a solution of double-stranded DNA is monitored at 260 nm as a...
One of the common methods to quantify DNA is to measure A260 (UV absorbance at 260 nm) of DNA solution using a spectrophotometer. You took 10 µl of your plasmid DNA preparation and dilute with 990 µl of H2O. The A260of this diluted solution was 0.30. Calculate the concentration of your plasmid DNA preparation (before the dilution). It has been established that 50 µg/ml of double-stranded DNA gives A260 = 1.0. My plasmid DNA preparation is _____________________ mg/ml. From this...
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