ZuoTi.Pro
Question

One of the common methods to quantify DNA is to measure A260 (UV absorbance at 260...

One of the common methods to quantify DNA is to measure A260 (UV absorbance at 260 nm) of DNA solution using a spectrophotometer. You took 10 µl of your plasmid DNA preparation and dilute with 990 µl of H2O. The A260of this diluted solution was 0.30. Calculate the concentration of your plasmid DNA preparation (before the dilution). It has been established that 50 µg/ml of double-stranded DNA gives A260 = 1.0.

My plasmid DNA preparation is _____________________ mg/ml.

From this I ended up with the answer 1.5 mg/ml.

I did 100*0.3*50 =1500 ug/ml => /1000(mg/ml) =1.5 mg/m

100= dilution factor

0.3= absorbance value

50= conversion factor

I just wanted to know that I went about this the right way or if i did something wrong?

science   chemistry   
0 0
Add a comment Improve this question Transcribed image text
Next > < Previous
Sort answers by oldest

Homework Answers

Request Professional Answer

Request Answer!

We need at least 10 more requests to produce the answer.

0 / 10 have requested this problem solution

The more requests, the faster the answer.

Request! (Login Required)

All students who have requested the answer will be notified once they are available.
Know the answer?
Add Answer to:
One of the common methods to quantify DNA is to measure A260 (UV absorbance at 260...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Similar Homework Help Questions

  • For a 1cm pathlength, the absorbance at 260nm (A260) equals 1.0 for a 50 microgram/mL solution...

    For a 1cm pathlength, the absorbance at 260nm (A260) equals 1.0 for a 50 microgram/mL solution of double stranded DNA. What is (i) the concentration and (ii) the amount of nucleic acid in 750 microlitre of a solution of double stranded DNA with an A260 of 0.46?

  • 18 15 16 17 7- What is the concentration of DNA whereby a 1:100 dilution has...

    18 15 16 17 7- What is the concentration of DNA whereby a 1:100 dilution has an observance reading of 0.015 at 260 nm? a. 6 ug mL b. 60 ug/mL c. 75 ug/mL d. 750 ug/mL 8- When measuring the concentration of RNA by spectrophotometry at 260 nm, the absorbance reading is multiplied by the dilution and a conversion factor of a. 20 6.30 c. 40 d. 50 9-DNA is isolated from a clinical sample. The absorbance at 260...

  • A student uses the same gel extraction and quantification protocols used in Lab 5, with the...

    A student uses the same gel extraction and quantification protocols used in Lab 5, with the following exceptions: Only half of the 50µL pMD2 plasmid digestion reaction was loaded on the gel. The student did a 1:25 dilution of the extracted backbone during the quantification. The student measures the same absorbance (0.015). Using the protocol form the lab manual, what was the concentration (in ng/µL) of the plasmid stock concentration before performing the digestion reaction? (2pts) Gel extraction Materials PE...

  • lab question 1. What is the basis of the different purification methods? 2. What are some...

    lab question 1. What is the basis of the different purification methods? 2. What are some of the factors the might have interfered with your results? 3. How might you improve the process to increase the yield and purity? lab process E. coli BL21 (DE3) cells were transformed with the pET Topo-1521 vector containing a reading frame encoding the green fluorescent protein (GFP). Cells were cultured in M9ZB media at 37°C until the absorbance at 600 nm reached 0.7, at...

  • Hi I am im a biochemistry lab and we are doing an experiment titled: PCR Amplification...

    Hi I am im a biochemistry lab and we are doing an experiment titled: PCR Amplification of LeuRS F C Terminal Domain. I am having troubles figuring out the rest of my caluclations. I already did the calculations for the first dilution set, I was wondering how I would do the calculation for the second dilution set. Also I was wondering how I would determine the theoretical values for my data. Is there a specific formula I can use? If...

  • I need the answers for questions 2 and 3. My DNA ladder is in lane 2...

    I need the answers for questions 2 and 3. My DNA ladder is in lane 2 marked by the yellow arrow. Thanks! Here is the only other info I have. Thanks! Part 2: Gel purification and on Gel Slice and PCR Product Preparin modified from TBSci.com instructions for gaan A. Dissolving the Gel Stie Following electrophores, eral DNA band from grand place glice microcentrifuge tube Ib. Use an analytical balance to weigh pelice Rec die 2. Add 500 balance to...

  • Please correct all questions because i did them wrong MATCHINGEnter the letter that best describes the...

    Please correct all questions because i did them wrong MATCHINGEnter the letter that best describes the reagent in the lefthand box below (12 Points) Answer G2 Buffer Proteinase K C1 Buffer Trypsin PBS VA. disrupts outer cell membrane of 3T3 fibroblasts B. phosphate buffered saline C. used to equilibrate Oiagen Genomic tip column D. precipitates DNA Eendonuclease, double stranded cleavage F. used to wash Qiagen Genomic tip column G. enzyme used to release 313 cells from culture task H. disrupts...

  • 2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented...

    2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented with arginine, methionine and the antibiotic tetracycline but lacking the essential amino acid isoleucine 3 How would you determine if your recombinant cells had also acquired the tryptophan gene from the Hart Complete the following table indicating which of the organisms (F and/or Hfr used in this experiment would be expected to grow on the given media. Unless specified, MMD is just minimal medium...

  • 1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteina...

    1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...

  • For the preparation and standardization of NaOH with KHP im supposed to boil water for 1hr and 30 min to remove CO2

    For the preparation and standardization of NaOH with KHP im supposed to boil water for 1hr and 30 min to remove CO2....the problem is that if I don't boil it for that long and (30 min) b/c of not enough time but I put the water I boiled for 1/2 hr aproximately into a NaOH bottle with a CO2 absorber and stored it there for a few days. I would assume that I would have to boil the water again...but...

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT