Calculate AE for the following reaction; Oxaloacetate + NADH Malate + NAD+ E'' (Oxaloacetate /malate) =...
Malate dehydrogenase catalyses the reaction L-malate + NAD+ <==> oxaloacetate + NADH + H+ and the following results were obtained, calculate Km and Vmax?
QUESTION 8 Consider the reaction: Malate + NAD → Oxaloacetate + NADH (AG'º = +29.7 kJ/mol) A cell at 25°C has the following concentrations of these compounds: malate = 0.5 mm oxaloacetate=0.00002 mM • NAD = 1 mM NADH = 0.04 mm . Is the reaction spontaneous? QUESTION 9 Consider the reaction: 1,3-Bisphosphoglycerate (1,3-BPG) + ADP→ 3-Phosphoglycerate (3-PG) + ATP (AG' = -18.9 kJ/mol) A cell at 25°C has the following concentrations of these compounds: 1,3-BPG = 0.003 Mm ADP...
For the reaction: L-Malate + NAD⁺ ← → Oxaloacetate + NADH ΔE' = -0.154 n=2 T=298 K using the equation ln (Keq) = (nFΔE'৹)/(RT) Find Keq? I know this sounds just plug and chug but am confused about the signs of the numbers when plugged in. Please only answer if you're of what you're doing! Standard Reduction Potential of Some Biologically Important Half-Reactions, at pH 7.0 and 25°C (298 K) Half-reaction E' (V) 2H+ + 2e — H, (at standard...
11.Calculate the standard free energy change for the oxidation of malate by NAD+ under physiological conditions. Given, (30 points) Malate «Oxaloacetate + 2H+ + 2e- Eo’ = 0.166 V NAD++ H+«2e-+ NADH Eo’ = - 0.315 V
4. According to standard redox potentials, oxaloacetate-malate couple is more positive than that of NAD+-NADH couple. Therefore, the oxidation of malate to oxaloacetate has a positive free energy change under standard conditions. In the cell, malate is oxidized to oxaloacetate. Explain how this is possible without the use of ATP. (2)
In the citric acid cycle, malate is dehydrogenated to oxaloacetate in a highly endergonic reaction with a ΔG’o of +30 kJ mol-1: L‐malate + NAD+ ⇌ oxaloacetate + NADH + H+ A. Calculate the equilibrium constant K’eq of this reaction. What is the implication of this result? B. Let us suppose that you have gathered two sets of data concerning the malate dehydrogenase reaction: (a) From one set of data obtained from three different model systems (rat, mouse and pig)...
Consider the malate dehydrogenase reaction from the citric acid cycle. Given the listed concentrations, calculate the free energy change for this reaction at energy change for this reaction at 37.0°C (310 K), AG'' for the reaction is +29.7 kJ/mol. Assume that the reaction occurs at pH 7. (malate) = 1.33 mm [oxaloacetate] = 0.200 mm [NAD) - 440 mm [NADH) - 180 mM AG: 36.9 KJ-mol-
Consider the malate dehydrogenase reaction from the citric acid cycle. Given the listed concentrations, calculate the free energy change for this reaction at energy change for this reaction at 37.0°C (310 K). AG' for the reaction is +29.7 kJ/mol. Assume that the reaction occurs at pH 7. [malate) = 1.43 mm [oxaloacetate) = 0.150 mM [NAD+] = 220 mm [NADH] = 88 mm kJ.mol-1 AG:
26. Based on our discussion in class, high levels of NADH would likely A. cause pellagra B. stimulate glycolysis C. slow down the movement of molecules through the citric acid cycle D. slow down oxidative phosphorylation E. break down carbohydrates 27. What purpose does fermentation serve? A. It regenerates NAD* from NADH to keep glycolysis going in the absence of oxygen. B. It allows for the production of NADH, which keeps the electron transport chain going. C. It produces an...
Consider the malate dehydrogenase reaction from the citric acid cycle. Given the listed concentrations, calculate the free energy change for this reaction at energy change for this reaction at 37.0 ∘ C (310 K). Δ G ∘ ′ for the reaction is + 29.7 kJ/mol . Assume that the reaction occurs at pH 7. thank you Consider the malate dehydrogenase reaction from the citric acid cycle. Given the listed concentrations, calculate the free energy change for this reaction at energy...