Question

4. Just by scanning through the amin 32-33 for the two methods of meas concentration of BSAP What about Ub? Explain. (10 pts) inning through the amino acid sequence, can you hypothesize what method (see page methods of measuring protein concentration) will be better at determining the 5. BSA stock is at 1 mg/ml. You want to make 200 ml 0.25 mg/ml BSA solution. How do you go about making this new BSA solution? (5 pts)

Answer 1

Given : - BSA stock Solution concentration = 1 mg/ml. Need to make zooml of 0.25 mg/ml concentration Now, i ml of stock contains ima 0.25 ml of stock will contain -0.25 mg. - 0.25 ml of stock solution + 0.75 ml of sel diluent solution (solvent/water) = l ml of solution with BSA concentration of o.25 mg Thus in order to make zooml, we need - 200x0.25 ml of stock solution + zooX 0.75 ml of diluent. 50ml of img/ml stock solution of BSA tisom of diluent (solvent or water CS Scazzed zimo ml of BSA solution of concentration o.25 mg/ml) Carb Scanner