Fall 2017 CHE 380: Introduction to Biotechnology Exam3 (I hr 20 min) Question 3 (28 polnts)...

Question

Question

Answer 1

Answer #1

a. The largest fragment is fluorescently tagged with ddCTP.

b. The sequence if we will read out will be TCAATGGTACTGTGA and its 3' end is T and 5' end is A.

c. The smallest fragment is fluorecently tagged with ddGTP.

d. The unknown template sequence is 3' GTAAGTGCTAGTTAC 5'.The 3' endof the template starts with G and the 5' end ends with C.

e. The mRNA transcript is 5' CAUUCACGAUCAAUG 3'.

f. The amino acid chain produced from DNA is histidine-serine-arginine-serine-methionone.

Answer 2

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