Question

Find the human BRCA1 gene transcript mRNA using NCBI refseq database.(hint: accession NM_007299.)

(a) What is the GI number of the protein?

(b) What is the length of the mRNA sequence?

(c) Write down the mRNA sequence as it is shown on the database.

(d) Also find and write the sequence in FASTA format.

(e) How many of each of the four nucleotides A, C, T and G, are there in the genome?

(f) How many occurrences of the DNA words CC, CG and GC occur in the genome?

(g) What are the last 50 nucleotides of the sequence?

(h) Provide the titles and Pubmed IDs (Pubmed ID is the number associated with the article and displayed after the title and Journal name in the annotations) of the First Three related Reference Journal Articles displayed?

(i) What does it say in the COMMENT field of its NCBI annotations record?

(j) Plot the Pie Chart representing frequencies of four nucleotides A, C, T and G in the genome

Answer 1

The GI number is 237681124

Length= 3,800 bp

mRNA-

CUUAGCGGUAGCCCCUUGGUUUCCGUGGCAACGGAAAAGCGCGGGAAUUACAGAUAAAUUAAAACUGCGACUG

CGCGGCGUGAGCUCGCUGAGACUUCCUGGACGGGGGACAGGCUGUGGGGUUUCUCAGAUAACUGGGCCCCU

GCGCUCAGGAGGCCUUCACCCUCUGCUCUGGUUCAUUGGAACAGAAAGAAAUGGAUUUAUCUGCUCUUCGCG

UUGAAGAAGUACAAAAUGUCAUUAAUGCUAUGCAGAAAAUCUUAGAGUGUCCCAUCUGUCUGGAGUUGAUCAA

GGAACCUGUCUCCACAAAGUGUGACCACAUAUUUUGCAAAUUUUGCAUGCUGAAACUUCUCAACCAGAAGAAA

GGGCCUUCACAGUGUCCUUUAUGUAAGAAUGAUAUAACCAAAAGGAGCCUACAAGAAAGUACGAGAUUUAGU

CAACUUGUUGAAGAGCUAUUGAAAAUCAUUUGUGCUUUUCAGCUUGACACAGGUUUGGAGUAUGCAAACAG

CUAUAAUUUUGCAAAAAAGGAAAAUAACUCUCCUGAACAUCUAAAAGAUGAAGUUUCUAUCAUCCAAAGUAU

GGGCUACAGAAACCGUGCCAAAAGACUUCUACAGAGUGAACCCGAAAAUCCUUCCUUGCAGGAAACCAGUC

UCAGUGUCCAACUCUCUAACCUUGGAACUGUGAGAACUCUGAGGACAAAGCAGCGGAUACAACCUCAAAAG

ACGUCUGUCUACAUUGAAUUGGGAUCUGAUUCUUCUGAAGAUACCGUUAAUAAGGCAACUUAUUGCAGUG

UGGGAGAUCAAGAAUUGUUACAAAUCACCCCUCAAGGAACCAGGGAUGAAAUCAGUUUGGAUUCUGCAAA

AAAGGCUGCUUGUGAAUUUUCUGAGACGGAUGUAACAAAUACUGAACAUCAUCAACCCAGUAAUAAUGAU

UUGAACACCACUGAGAAGCGUGCAGCUGAGAGGCAUCCAGAAAAGUAUCAGGGUGAAGCAGCAUCUGGG

UGUGAGAGUGAAACAAGCGUCUCUGAAGACUGCUCAGGGCUAUCCUCUCAGAGUGACAUUUUAACCACU

CAGCAGAGGGAUACCAUGCAACAUAACCUGAUAAAGCUCCAGCAGGAAAUGGCUGAACUAGAAGCUGUGU

UAGAACAGCAUGGGAGCCAGCCUUCUAACAGCUACCCUUCCAUCAUAAGUGACUCUUCUGCCCUUGAGG

ACCUGCGAAAUCCAGAACAAAGCACAUCAGAAAAAGUAUUAACUUCACAGAAAAGUAGUGAAUACCCUAUA

AGCCAGAAUCCAGAAGGCCUUUCUGCUGACAAGUUUGAGGUGUCUGCAGAUAGUUCUACCAGUAAAAAU

AAAGAACCAGGAGUGGAAAGGUCAUCCCCUUCUAAAUGCCCAUCAUUAGAUGAUAGGUGGUACAUGCACA

GUUGCUCUGGGAGUCUUCAGAAUAGAAACUACCCAUCUCAAGAGGAGCUCAUUAAGGUUGUUGAUGUGG

AGGAGCAACAGCUGGAAGAGUCUGGGCCACACGAUUUGACGGAAACAUCUUACUUGCCAAGGCAAGAUC

UAGAGGGAACCCCUUACCUGGAAUCUGGAAUCAGCCUCUUCUCUGAUGACCCUGAAUCUGAUCCUUCUG

AAGACAGAGCCCCAGAGUCAGCUCGUGUUGGCAACAUACCAUCUUCAACCUCUGCAUUGAAAGUUCCCCA

AUUGAAAGUUGCAGAAUCUGCCCAGAGUCCAGCUGCUGCUCAUACUACUGAUACUGCUGGGUAUAAUGC

AAUGGAAGAAAGUGUGAGCAGGGAGAAGCCAGAAUUGACAGCUUCAACAGAAAGGGUCAACAAAAGAAUG

UCCAUGGUGGUGUCUGGCCUGACCCCAGAAGAAUUUAUGCUCGUGUACAAGUUUGCCAGAAAACACCAC

AUCACUUUAACUAAUCUAAUUACUGAAGAGACUACUCAUGUUGUUAUGAAAACAGAUGCUGAGUUUGUGU

GUGAACGGACACUGAAAUAUUUUCUAGGAAUUGCGGGAGGAAAAUGGGUAGUUAGCUAUUUCUGGGUGA

CCCAGUCUAUUAAAGAAAGAAAAAUGCUGAAUGAGCAUGAUUUUGAAGUCAGAGGAGAUGUGGUCAAUGG

AAGAAACCACCAAGGUCCAAAGCGAGCAAGAGAAUCCCAGGACAGAAAGAUCUUCAGGGGGCUAGAAAUC

UGUUGCUAUGGGCCCUUCACCAACAUGCCCACAGGGUGUCCACCCAAUUGUGGUUGUGCAGCCAGAUGC

CUGGACAGAGGACAAUGGCUUCCAUGCAAUUGGGCAGAUGUGUGAGGCACCUGUGGUGACCCGAGAGU

GGGUGUUGGACAGUGUAGCACUCUACCAGUGCCAGGAGCUGGACACCUACCUGAUACCCCAGAUCCCCC

ACAGCCACUACUGACUGCAGCCAGCCACAGGUACAGAGCCACAGGACCCCAAGAAUGAGCUUACAAAGUG

GCCUUUCCAGGCCCUGGGAGCUCCUCUCACUCUUCAGUCCUUCUACUGUCCUGGCUACUAAAUAUUUUA

UGUACAUCAGCCUGAAAAGGACUUCUGGCUAUGCAAGGGUCCCUUAAAGAUUUUCUGCUUGAAGUCUCC

CUUGGAAAUCUGCCAUGAGCACAAAAUUAUGGUAAUUUUUCACCUGAGAAGAUUUUAAAACCAUUUAAAC

GCCACCAAUUGAGCAAGAUGCUGAUUCAUUAUUUAUCAGCCCUAUUCUUUCUAUUCAGGCUGUUGUUGG

CUUAGGGCUGGAAGCACAGAGUGGCUUGGCCUCAAGAGAAUAGCUGGUUUCCCUAAGUUUACUUCUCUA

AAACCCUGUGUUCACAAAGGCAGAGAGUCAGACCCUUCAAUGGAAGGAGAGUGCUUGGGAUCGAUUAUG

UGACUUAAAGUCAGAAUAGUCCUUGGGCAGUUCUCAAAUGUUGGAGUGGAACAUUGGGGAGGAAAUUCU

GAGGCAGGUAUUAGAAAUGAAAAGGAAACUUGAAACCUGGGCAUGGUGGCUCACGCCUGUAAUCCCAGC

ACUUUGGGAGGCCAAGGUGGGCAGAUCACUGGAGGUCAGGAGUUCGAAACCAGCCUGGCCAACAUGGU

GAAACCCCAUCUCUACUAAAAAUACAGAAAUUAGCCGGUCAUGGUGGUGGACACCUGUAAUCCCAGCUAC

UCAGGUGGCUAAGGCAGGAGAAUCACUUCAGCCCGGGAGGUGGAGGUUGCAGUGAGCCAAGAUCAUA

CCACGGCACUCCAGCCUGGGUGACAGUGAGACUGUGGCUCAAAAAAAAAAAAAAAAAAAGGAAAAUGA

AACUAGAAGAGAUUUCUAAAAGUCUGAGAUAUAUUUGCUAGAUUUCUAAAGAAUGUGUUCUAAAACAG

CAGAAGAUUUUCAAGAACCGGUUUCCAAAGACAGUCUUCUAAUUCCUCAUUAGUAAUAAGUAAAAUGU

UUAUUGUUGUAGCUCUGGUAUAUAAUCCAUUCCUCUUAAAAUAUAAGACCUCUGGCAUGAAUAUUUCA

UAUCUAUAAAAUGACAGAUCCCACCAGGAAGGAAGCUGUUGCUUUCUUUGAGGUGAUUUUUUUCCUU

UGCUCCCUGUUGCUGAAACCAUACAGCUUCAUAAAUAAUUUUGCUUGCUGAAGGAAGAAAAAGUGUU

UUUCAUAAACCCAUUAUCCAGGACUGUUUAUAGCUGUUGGAAGGACUAGGUCUUCCCUAGCCCCCCC

AGUGUGCAAGGGCAGUGAAGACUUGAUUGUACAAAAUACGUUUUGUAAAUGUUGUGCUGUUAACACU

GCAAAUAAACUUGGUAGCAAACACUUCCAAAAAAAAAAAAAAAAAA

FastA_

CTTAGCGGTAGCCCCTTGGTTTCCGTGGCAACGGAAAAGCGCGGGAATTACAGATAAATTAAAACTGCGA
CTGCGCGGCGTGAGCTCGCTGAGACTTCCTGGACGGGGGACAGGCTGTGGGGTTTCTCAGATAACTGGGC
CCCTGCGCTCAGGAGGCCTTCACCCTCTGCTCTGGTTCATTGGAACAGAAAGAAATGGATTTATCTGCTC
TTCGCGTTGAAGAAGTACAAAATGTCATTAATGCTATGCAGAAAATCTTAGAGTGTCCCATCTGTCTGGA
GTTGATCAAGGAACCTGTCTCCACAAAGTGTGACCACATATTTTGCAAATTTTGCATGCTGAAACTTCTC
AACCAGAAGAAAGGGCCTTCACAGTGTCCTTTATGTAAGAATGATATAACCAAAAGGAGCCTACAAGAAA
GTACGAGATTTAGTCAACTTGTTGAAGAGCTATTGAAAATCATTTGTGCTTTTCAGCTTGACACAGGTTT
GGAGTATGCAAACAGCTATAATTTTGCAAAAAAGGAAAATAACTCTCCTGAACATCTAAAAGATGAAGTT
TCTATCATCCAAAGTATGGGCTACAGAAACCGTGCCAAAAGACTTCTACAGAGTGAACCCGAAAATCCTT
CCTTGCAGGAAACCAGTCTCAGTGTCCAACTCTCTAACCTTGGAACTGTGAGAACTCTGAGGACAAAGCA
GCGGATACAACCTCAAAAGACGTCTGTCTACATTGAATTGGGATCTGATTCTTCTGAAGATACCGTTAAT
AAGGCAACTTATTGCAGTGTGGGAGATCAAGAATTGTTACAAATCACCCCTCAAGGAACCAGGGATGAAA
TCAGTTTGGATTCTGCAAAAAAGGCTGCTTGTGAATTTTCTGAGACGGATGTAACAAATACTGAACATCA
TCAACCCAGTAATAATGATTTGAACACCACTGAGAAGCGTGCAGCTGAGAGGCATCCAGAAAAGTATCAG
GGTGAAGCAGCATCTGGGTGTGAGAGTGAAACAAGCGTCTCTGAAGACTGCTCAGGGCTATCCTCTCAGA
GTGACATTTTAACCACTCAGCAGAGGGATACCATGCAACATAACCTGATAAAGCTCCAGCAGGAAATGGC
TGAACTAGAAGCTGTGTTAGAACAGCATGGGAGCCAGCCTTCTAACAGCTACCCTTCCATCATAAGTGAC
TCTTCTGCCCTTGAGGACCTGCGAAATCCAGAACAAAGCACATCAGAAAAAGTATTAACTTCACAGAAAA
GTAGTGAATACCCTATAAGCCAGAATCCAGAAGGCCTTTCTGCTGACAAGTTTGAGGTGTCTGCAGATAG
TTCTACCAGTAAAAATAAAGAACCAGGAGTGGAAAGGTCATCCCCTTCTAAATGCCCATCATTAGATGAT
AGGTGGTACATGCACAGTTGCTCTGGGAGTCTTCAGAATAGAAACTACCCATCTCAAGAGGAGCTCATTA
AGGTTGTTGATGTGGAGGAGCAACAGCTGGAAGAGTCTGGGCCACACGATTTGACGGAAACATCTTACTT
GCCAAGGCAAGATCTAGAGGGAACCCCTTACCTGGAATCTGGAATCAGCCTCTTCTCTGATGACCCTGAA
TCTGATCCTTCTGAAGACAGAGCCCCAGAGTCAGCTCGTGTTGGCAACATACCATCTTCAACCTCTGCAT
TGAAAGTTCCCCAATTGAAAGTTGCAGAATCTGCCCAGAGTCCAGCTGCTGCTCATACTACTGATACTGC
TGGGTATAATGCAATGGAAGAAAGTGTGAGCAGGGAGAAGCCAGAATTGACAGCTTCAACAGAAAGGGTC
AACAAAAGAATGTCCATGGTGGTGTCTGGCCTGACCCCAGAAGAATTTATGCTCGTGTACAAGTTTGCCA
GAAAACACCACATCACTTTAACTAATCTAATTACTGAAGAGACTACTCATGTTGTTATGAAAACAGATGC
TGAGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAATTGCGGGAGGAAAATGGGTAGTTAGCTAT
TTCTGGGTGACCCAGTCTATTAAAGAAAGAAAAATGCTGAATGAGCATGATTTTGAAGTCAGAGGAGATG
TGGTCAATGGAAGAAACCACCAAGGTCCAAAGCGAGCAAGAGAATCCCAGGACAGAAAGATCTTCAGGGG
GCTAGAAATCTGTTGCTATGGGCCCTTCACCAACATGCCCACAGGGTGTCCACCCAATTGTGGTTGTGCA
GCCAGATGCCTGGACAGAGGACAATGGCTTCCATGCAATTGGGCAGATGTGTGAGGCACCTGTGGTGACC
CGAGAGTGGGTGTTGGACAGTGTAGCACTCTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGA
TCCCCCACAGCCACTACTGACTGCAGCCAGCCACAGGTACAGAGCCACAGGACCCCAAGAATGAGCTTAC
AAAGTGGCCTTTCCAGGCCCTGGGAGCTCCTCTCACTCTTCAGTCCTTCTACTGTCCTGGCTACTAAATA
TTTTATGTACATCAGCCTGAAAAGGACTTCTGGCTATGCAAGGGTCCCTTAAAGATTTTCTGCTTGAAGT
CTCCCTTGGAAATCTGCCATGAGCACAAAATTATGGTAATTTTTCACCTGAGAAGATTTTAAAACCATTT
AAACGCCACCAATTGAGCAAGATGCTGATTCATTATTTATCAGCCCTATTCTTTCTATTCAGGCTGTTGT
TGGCTTAGGGCTGGAAGCACAGAGTGGCTTGGCCTCAAGAGAATAGCTGGTTTCCCTAAGTTTACTTCTC
TAAAACCCTGTGTTCACAAAGGCAGAGAGTCAGACCCTTCAATGGAAGGAGAGTGCTTGGGATCGATTAT
GTGACTTAAAGTCAGAATAGTCCTTGGGCAGTTCTCAAATGTTGGAGTGGAACATTGGGGAGGAAATTCT
GAGGCAGGTATTAGAAATGAAAAGGAAACTTGAAACCTGGGCATGGTGGCTCACGCCTGTAATCCCAGCA
CTTTGGGAGGCCAAGGTGGGCAGATCACTGGAGGTCAGGAGTTCGAAACCAGCCTGGCCAACATGGTGAA
ACCCCATCTCTACTAAAAATACAGAAATTAGCCGGTCATGGTGGTGGACACCTGTAATCCCAGCTACTCA
GGTGGCTAAGGCAGGAGAATCACTTCAGCCCGGGAGGTGGAGGTTGCAGTGAGCCAAGATCATACCACGG
CACTCCAGCCTGGGTGACAGTGAGACTGTGGCTCAAAAAAAAAAAAAAAAAAAGGAAAATGAAACTAGAA
GAGATTTCTAAAAGTCTGAGATATATTTGCTAGATTTCTAAAGAATGTGTTCTAAAACAGCAGAAGATTT
TCAAGAACCGGTTTCCAAAGACAGTCTTCTAATTCCTCATTAGTAATAAGTAAAATGTTTATTGTTGTAG
CTCTGGTATATAATCCATTCCTCTTAAAATATAAGACCTCTGGCATGAATATTTCATATCTATAAAATGA
CAGATCCCACCAGGAAGGAAGCTGTTGCTTTCTTTGAGGTGATTTTTTTCCTTTGCTCCCTGTTGCTGAA
ACCATACAGCTTCATAAATAATTTTGCTTGCTGAAGGAAGAAAAAGTGTTTTTCATAAACCCATTATCCA
GGACTGTTTATAGCTGTTGGAAGGACTAGGTCTTCCCTAGCCCCCCCAGTGTGCAAGGGCAGTGAAGACT
TGATTGTACAAAATACGTTTTGTAAATGTTGTGCTGTTAACACTGCAAATAAACTTGGTAGCAAACACTT
CCAAAAAAAAAAAAAAAAAA