Question

Attachment.

What is the name of the enzyme that inactivates cyclic adenosine monophosphate? Based on the table shown, what were the units of measurement for cyclic AMP? 'source: http//www.pnas.org/content/71M0/3844.full pod +html TABLE 4. Correlation between phoephodiesterase activity and refractorinesa of the cells to a aeoond morepineplirine treatment CAMP phoepbodiesterase eyelie AMP High activity 10-min Low K- activity (pmoles/mg of protein per min (pmoles/mg of protein) 82.8 1.6 360 30 12.2 t 1.0 iDeubstion 1711 203 NE 78.1 th 6.6 701 49 57.2 7.1 NE 265 at 12 NE NE 57.4 10.9 42.0 1.7 NE CH 416 49 1486 141 NE CH NE 115 NE After removal of old medium, the oells were wwhed two times with Hanks' balanoed salta alation and inoubated in 3 ml of Ham's F-10 at 37 Norepinephrine (NE) was present at 0.1 mM and eyelobeximide (CH) at 25 At the of 4-br the medium was removed, the eells were washed once, and 3 ml of medium containing the indieaued were added for a second 10 min incubation, Cyelie AMP and phoephodienterase were determined as deneribed under Materials and Methods, The resulta represent the mean SEM (n 4 for phosphodiesterases 3 for eyelie AMP) The experiment was repeated three times

Answer 1

Phosphodiesterase is an enzyme which inactivates cAMP. Units of measurements for cyclicAMP are pmoles/mg.