Question

Look at the immunohistochemical fluorescent tagged picture below. In this preparation multiple proteins were "tagged" using antibodies or chemicals that were conjugated to fluorescent tags. The blue stain is DAPI which binds to adenosine/tyrosine rich areas of DNA. The green stain identifies microtubules. You will notice that unlike H and E preparations you can't see any other structures within the cell. Adjacent to the cell of interest there are multiple nuclei visible which show diffuse and unorganized DNA. What stage of cell division would you predict the center cell to be in? Justify your answer.

Answer 1

The Cell is most probably in metaphase of cell division. This is beacause all the genetic material(pink fluorescent structures) is lying in the middle of the cell as seen in metaphase plate.