For reference-
Example Ponceau S Stained nitrocellulose membrane (NCF)
Samples in each lane
1: SeeBlue 2 Prestrained Protein Markers
2: Purified IgY from egg yolk
3: Bacterial cell extract (E. coli cell extract)
4: Mammalian cell extract (Ox heart cell extract)
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For reference-
Example Ponceau S Stained nitrocellulose membrane (NCF)
Samples in each lane
1: SeeBlue 2...
can anyone help me with this cell bio question? In Figure 2 part A (Non-denaturing). Lane 1 NEP( E403C) , 2 = WT...
can anyone help me with this cell bio
question?
In Figure 2 part A (Non-denaturing). Lane 1 NEP( E403C) , 2 = WT ECE, , 4= WT= NEP, 9= ECE(C416E). Figure 2 part B. The same as part A, but under denaturing conditions. (note; for simplicity, many lanes are ignored) 1 2 3 5 67 89 10 a -213 -123 -85 -50 b kDa -213 -123 85 -50 Figure 2 Immunoblot analyses of wild-type and cysteine mulants of NEP and...
Prac result Nhel Kpn I 1569 1601 Kpni 1030 2295 / 2310 Not I 988 ОТС-д...
Prac result
Nhel Kpn I 1569 1601 Kpni 1030 2295 / 2310 Not I 988 ОТС-д Sphi 2559 Nde I 484 POTC-A 6401 bp Hygro Amp Nde1 3369 pUC Ori 5260 4587 Amp: Ampicillin resistance gene 5405 - 6265 Hygro: Hygromycin resistance gene Choose the gel image with correct bands in lanes 1 to 5 to indicate the approximate positions where you expect to see these predicted DNA fragments. (1 mark) Lane 1: POTC (undigested / uncut) Lane 2: POTC-A...
1. Like phospholipids, lipopolysaccharides have a hydrophilic head and a hydrophobic tail. True or false 2....
1. Like phospholipids, lipopolysaccharides have a hydrophilic head and a hydrophobic tail. True or false 2. The outer membrane of Gram negative bacteria contains lipopolysaccharides T or F 3. Biofilms and pure culture are formed by only one type of microorganism T or F 4. Any given antibiotic will have the same minimum inhibitory concentration (MIC) against any test microorganism. T or F 5. Production of antibiotics by the microorganism is not affected by the culture conditions T or F...
The Jounal of Immunol nes of the National Academy of Sciences USA 89. 6550-6554. 01992, by permis...
explain figure 2 please on the third page
The Jounal of Immunol nes of the National Academy of Sciences USA 89. 6550-6554. 01992, by permision of Proc. Natl. Acad. Sci. USA Vol. 89, pp. 6550-63554, July 1992 Immunolog A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells RANDOLPH J. NOELLE时, MEENAKSHI ROY.. DAVID M. SHEPHERD., IVAN STAMENKOVICt JEFFREY A. LEDBETTER, AND ALEJANDRO ARUFFo Departmend Microbiology. Dartmouth Medical School One...
strand(s) and the RNA molecule is made up of strand(s). The DNA molecule is made up...
strand(s) and the RNA molecule is made up of strand(s). The DNA molecule is made up of O a. 4; 2 Ob. 1; 1 O c. 2; 1 O d. 1; 2 O e. 2; 4 QUESTION 45 The formation of large molecules from small subunits is known as what kind of reaction? O a reduction O b.condensation Oc decarboxylation d. hydrolysis O e. oxidation What is formed when an atom loses or gains an electron? O a. ion b....
BACKGROUND: Mycobacterium tuberculosis causes tuberculosis, one of the world’s deadliest diseases...
BACKGROUND:
Mycobacterium tuberculosis causes tuberculosis, one of the world’s
deadliest diseases. It is an acid-fast bacillus that is 2-4 um in
length and 0.2-0.5 um in width.
How many weeks, days,& hours would it take to see a
M.tuberculosis colony on solid media? Its generation time is listed
in the reading (above). Assume that bacterial colonies are not
visible to the naked eye unless they contain ≥ 2 millioncells. This
species forms aggregates of 5 cells on average. Show your...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...
1. According to the paper, what does lactate dehydrogenase (LDH) do and what does it allow...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
can anyone help me with this cell bio
question?
In Figure 2 part A (Non-denaturing). Lane 1 NEP( E403C) , 2 = WT ECE, , 4= WT= NEP, 9= ECE(C416E). Figure 2 part B. The same as part A, but under denaturing conditions. (note; for simplicity, many lanes are ignored) 1 2 3 5 67 89 10 a -213 -123 -85 -50 b kDa -213 -123 85 -50 Figure 2 Immunoblot analyses of wild-type and cysteine mulants of NEP and...
Prac result
Nhel Kpn I 1569 1601 Kpni 1030 2295 / 2310 Not I 988 ОТС-д Sphi 2559 Nde I 484 POTC-A 6401 bp Hygro Amp Nde1 3369 pUC Ori 5260 4587 Amp: Ampicillin resistance gene 5405 - 6265 Hygro: Hygromycin resistance gene Choose the gel image with correct bands in lanes 1 to 5 to indicate the approximate positions where you expect to see these predicted DNA fragments. (1 mark) Lane 1: POTC (undigested / uncut) Lane 2: POTC-A...
explain figure 2 please on the third page
The Jounal of Immunol nes of the National Academy of Sciences USA 89. 6550-6554. 01992, by permision of Proc. Natl. Acad. Sci. USA Vol. 89, pp. 6550-63554, July 1992 Immunolog A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells RANDOLPH J. NOELLE时, MEENAKSHI ROY.. DAVID M. SHEPHERD., IVAN STAMENKOVICt JEFFREY A. LEDBETTER, AND ALEJANDRO ARUFFo Departmend Microbiology. Dartmouth Medical School One...
strand(s) and the RNA molecule is made up of strand(s). The DNA molecule is made up of O a. 4; 2 Ob. 1; 1 O c. 2; 1 O d. 1; 2 O e. 2; 4 QUESTION 45 The formation of large molecules from small subunits is known as what kind of reaction? O a reduction O b.condensation Oc decarboxylation d. hydrolysis O e. oxidation What is formed when an atom loses or gains an electron? O a. ion b....
BACKGROUND:
Mycobacterium tuberculosis causes tuberculosis, one of the world’s
deadliest diseases. It is an acid-fast bacillus that is 2-4 um in
length and 0.2-0.5 um in width.
How many weeks, days,& hours would it take to see a
M.tuberculosis colony on solid media? Its generation time is listed
in the reading (above). Assume that bacterial colonies are not
visible to the naked eye unless they contain ≥ 2 millioncells. This
species forms aggregates of 5 cells on average. Show your...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
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