Homework Answers
The antibiotic resistant genes present in the plasmid used as selection marker. When bacterial cells are transformed by using the plasmid carrying the genes of interest some bacterial cells takes the plasmid successfully while some bacterial cells does not take the plasmid. Now the antibiotic resiatant gene present in the plasmid is used as selection marker. The cells after transformation cultured in the the media having antibiotic. Now those cells which have taken the plasmid, have the antibiotic resistant genes and are able to grow in the culture media while those cells which have not taken the plasmid will not have the antibiotic resistant gene and will die. So only transformed cell will grow in culture media. In this way antibiotics genes present in the plasmid used as a selection marker.
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Many plasmids used to express recombinant protein also carry a gene which codes for resistance to...
QUESTION 5 A protein is involved in gene expression. You study it and fins that it...
QUESTION 5 A protein is involved in gene expression. You study it and fins that it has a Helix turn helix domain. What is the protein likely to be (ie, what does a helix turn helix domain indicate)? TTT Arial 3(12pt) T.E.E. O S Path:p Words:0 QUESTION 6 You find that when you remove this molecule from the cells the expression of the gene it regulates rises. Is this element a repressor or an activator? TT T Arial - 3...
plz help the inoculating loop After you use an inoculating loop to transfer bacteria from a...
plz help
the inoculating loop After you use an inoculating loop to transfer bacteria from a culture tube onto a slide, you should before putting it away For the toolbar, press ALT+F10 (PC) or ALT-FN-F10 (Mac). TTTT Paragraph Arial 3 (12pt) E - T %D0Q TT. Пнти се QIX I Path: P Words:0 QUESTION 3 In lab, bacterial cultures (Petri plates and tubes of culture broth) should be kept covered at all times. This is partly to protect us from...
Using Java, Class Invoice could be used by a hardware store to represent an invoice for...
Using Java,
Class Invoice could be used by a hardware store to represent an invoice for an item sold at the store. An Invoice should include four pieces of information as instance variables—a part number, a part description, a quantity of the item being purchased, and a price per item. Code the class with the instance variables and a constructor for the class that initializes the four instance variables with values received by parameters of the same name. TT T...
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been...
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been isolated and purified, but its amino acid sequence has not been determined. We wish to clone the gene for protein P. (a) How can a probe be prepared to identify the gene for protein P? (b) If we have prepared a radioactive messenger RNA as our probe in part (a), how could we verify that it is the mRNA for protein P? (c) If...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
QUESTION The rough-skinned newt, Taricha granulosa, is a common prey species for garter snakes, Thamnophis sirtalis....
QUESTION The rough-skinned newt, Taricha granulosa, is a common prey species for garter snakes, Thamnophis sirtalis. odotoxin biosynthes s in newts is poary understocd, but its mechanism of action is known: the toxin binds to the Voltage-0ated Na channel found on the membrane of nerve cells and bigcks actico pctentias in nerves and muscles Garter snakes that prey on newts become poisoned if they eat TTX-producing newts. However, resistance to TTX has evolved in some garter the votage-gated sodium channel...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
i need all 5 parts of question QUESTION 1 What is your wish? Think about your...
i need all 5 parts of question
QUESTION 1 What is your wish? Think about your goals, wishes, and concerns. Pick a wish or goal that feels challenging but that you can reasonably fulfill within the next few weeks. Write a paragraph describing this goal and why it is important to you. For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac). QUESTION 2 What is the best outcome? If your wish is fulfilled, where would that leave you? What would...
QUESTION 6 Assume you are studying a protein-coding gene, ACEX, which includes 4 exons as illustrated...
QUESTION 6 Assume you are studying a protein-coding gene, ACEX, which includes 4 exons as illustrated in the gene map below. The 5' UTR and 3' UTR segments are each 25 bp long. Exons 1 thru 4 are 100, 200, 300, 400 bp long, respectively. Each intron is 200 bp each. The locations of the relevant EcoRI sites within the ACEX locus are indicated, but the location of other restriction enzyme sites (like BamHI) are not shown." EcoRI probe EcoRI...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented with arginine, methionine and the antibiotic tetracycline but lacking the essential amino acid isoleucine 3 How would you determine if your recombinant cells had also acquired the tryptophan gene from the Hart Complete the following table indicating which of the organisms (F and/or Hfr used in this experiment would be expected to grow on the given media. Unless specified, MMD is just minimal medium...
QUESTION 5 A protein is involved in gene expression. You study it and fins that it has a Helix turn helix domain. What is the protein likely to be (ie, what does a helix turn helix domain indicate)? TTT Arial 3(12pt) T.E.E. O S Path:p Words:0 QUESTION 6 You find that when you remove this molecule from the cells the expression of the gene it regulates rises. Is this element a repressor or an activator? TT T Arial - 3...
plz help
the inoculating loop After you use an inoculating loop to transfer bacteria from a culture tube onto a slide, you should before putting it away For the toolbar, press ALT+F10 (PC) or ALT-FN-F10 (Mac). TTTT Paragraph Arial 3 (12pt) E - T %D0Q TT. Пнти се QIX I Path: P Words:0 QUESTION 3 In lab, bacterial cultures (Petri plates and tubes of culture broth) should be kept covered at all times. This is partly to protect us from...
Using Java,
Class Invoice could be used by a hardware store to represent an invoice for an item sold at the store. An Invoice should include four pieces of information as instance variables—a part number, a part description, a quantity of the item being purchased, and a price per item. Code the class with the instance variables and a constructor for the class that initializes the four instance variables with values received by parameters of the same name. TT T...
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been isolated and purified, but its amino acid sequence has not been determined. We wish to clone the gene for protein P. (a) How can a probe be prepared to identify the gene for protein P? (b) If we have prepared a radioactive messenger RNA as our probe in part (a), how could we verify that it is the mRNA for protein P? (c) If...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
QUESTION The rough-skinned newt, Taricha granulosa, is a common prey species for garter snakes, Thamnophis sirtalis. odotoxin biosynthes s in newts is poary understocd, but its mechanism of action is known: the toxin binds to the Voltage-0ated Na channel found on the membrane of nerve cells and bigcks actico pctentias in nerves and muscles Garter snakes that prey on newts become poisoned if they eat TTX-producing newts. However, resistance to TTX has evolved in some garter the votage-gated sodium channel...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
i need all 5 parts of question
QUESTION 1 What is your wish? Think about your goals, wishes, and concerns. Pick a wish or goal that feels challenging but that you can reasonably fulfill within the next few weeks. Write a paragraph describing this goal and why it is important to you. For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac). QUESTION 2 What is the best outcome? If your wish is fulfilled, where would that leave you? What would...
QUESTION 6 Assume you are studying a protein-coding gene, ACEX, which includes 4 exons as illustrated in the gene map below. The 5' UTR and 3' UTR segments are each 25 bp long. Exons 1 thru 4 are 100, 200, 300, 400 bp long, respectively. Each intron is 200 bp each. The locations of the relevant EcoRI sites within the ACEX locus are indicated, but the location of other restriction enzyme sites (like BamHI) are not shown." EcoRI probe EcoRI...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented with arginine, methionine and the antibiotic tetracycline but lacking the essential amino acid isoleucine 3 How would you determine if your recombinant cells had also acquired the tryptophan gene from the Hart Complete the following table indicating which of the organisms (F and/or Hfr used in this experiment would be expected to grow on the given media. Unless specified, MMD is just minimal medium...
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