Question

days, depending on Therefore, three larval periods of growh, callled between molts. When fully grown, Dhe thind-stage inatar larvae cease feeding and pupale. During pupatlion, which lasts about four days, the larval tissues are reorganized to form those of the 1. Pat as or am Use a s mcn 2. 1M you ae lockin view the es Eamine wd body, and shom 4. Examine matm adult. The life cycle is summarized in Flgare 9.3. Figure 9.3 Life cycle of the fruit ly, showing differences between adult sexes Male has ses combe amae acs seoms Table 9.2 Chu wg lengh Calor of eyes Caler of body S. Use the The m The m Dosa 10-14 days O vertral dark Fertied egg Vent Pupa Oven 1 day this 6. In anl of ch and the thr First inatar larve Third instar larva 3-5 day Figure in Dros have 0-6 Second instar larve Observation: Drosophila melanogaster You will be provided with a stock culture of Drosophila melanogaster to examine. Answer the following questions: 1. Where in the culture vial are the adult flies? 2. The eggs? Culture 3. The larvae? 4. The pupae? 9-4 Mendelian Genetics Laboratory 9 106 O0

Answer 1

Drophilla melanogasta exibits complete metamorphism meaning the life cycle includes an egg, larvel form, pupa and finally emergence as the flying adult. This is the same as the well known metamorphosis of butterflies. The larvel stage has three in steps or molts

Domain - Eukarya

Kingdom -. Animalia

Phylum - Arthropods

Class - Insecta

Order - Drosophila

Geneus - Drosophila

Species - Melanogasta

1.

* Individual culture

* F1 culture

* Easy fly culture

2. Eggs

* Females can lay up to100 eggs / day

* Vergin femails are able to lay eggs. However they will be sterile and few in number.

Larvae

After the egg hatch small larvae should be visible in the growing medium.lf your media is white cook for the small black area at the head of the larvae. Some dried premixed media is blue to help identify larvae how ever this is not a necessity and with and little patience and practices larvae are easily Sean. In addition as the larvae feed they disrupt the smooth surface of the media and soby looking only at the surface one can tell if larvae are present.

Pupae

The larvae will begin to migrate up the culture vid in order to pupate.