The region of FK1 needed for nuclear export has a motif LRxSxSTxTxP with several serine and...
The region of FK1 needed for nuclear export has a motif LRxSxSTxTxP with several serine and threonine residues (x = any amino acid).
Briefly describe an experiment that would test if any of these residues is a target for protein phosphorylation. (3 pts)
Homework Answers
Test for weather the protein has some site for phosphorylation ot not can be tested by site directed phosphorylation in each of these specific amino acid residue then followed by treatment with kinase enzyme which can abke to incorporate phosphate group the phosphate must be tagged with Radiolabbled which can be identified by autoradiography.
If the amino acid sequence is a target form phosphorylation then the Radiolabbled phosphate cannot be identified as as other amino acid residue are blocked with phosphorylation. So,Radiolabbled sigbal will be observed. Thus, phosphorylation inhibition assay can be used for detection of site specific phosphorylation withen a sequence of a protein
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The region of FK1 needed for nuclear export has a motif
LRxSxSTxTxP with several serine and...
Given the information provided, would curve 1 or 2 in the graph correspond to the serine...
Given the information provided, would curve 1 or 2 in the
graph correspond to the serine residue? (There is no more
additional infirmation besides what is given in the problem and the
graph)
Consider a signaling protein that can be phosphorylated by a dual-specificity protein kinase at two independent sites (one tyrosine and one serine residue), such that each site is phosphorylated in about 1% of the protein molecules under normal conditions Phosphorylation at the first site has a short...
18. A DNA-binding protein is needed to be expressed and purified for structural studies. It has...
18. A DNA-binding protein is needed to be expressed and purified for structural studies. It has the following properties: (a) It is a human protein; (b) The overall molecular mass is about 150 kDa; (c) It contains rare codons; (d) It has multiple charged and hydrophobic amino acid residues; (e) Its pI is 5.4; It is sensitive to proteolytic degradation. Starting from the design for protein expression using an appropriate expression system, briefly describe how you would proceed logically to...
1) (10 pts) Serine proteases are enzymes that cleave peptide bonds in proteins. Explain using words...
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Given the information provided, would curve 1 or 2 in the
graph correspond to the serine residue? (There is no more
additional infirmation besides what is given in the problem and the
graph)
Consider a signaling protein that can be phosphorylated by a dual-specificity protein kinase at two independent sites (one tyrosine and one serine residue), such that each site is phosphorylated in about 1% of the protein molecules under normal conditions Phosphorylation at the first site has a short...
18. A DNA-binding protein is needed to be expressed and purified for structural studies. It has the following properties: (a) It is a human protein; (b) The overall molecular mass is about 150 kDa; (c) It contains rare codons; (d) It has multiple charged and hydrophobic amino acid residues; (e) Its pI is 5.4; It is sensitive to proteolytic degradation. Starting from the design for protein expression using an appropriate expression system, briefly describe how you would proceed logically to...
1) (10 pts) Serine proteases are enzymes that cleave peptide bonds in proteins. Explain using words (drawings OR both) why serine proteases cleave either before or after different amino acid residues. Talk about at least two of the following proteases: Chymotrypsin, Trypsin, or Elastase. mod 2) (10pts) Below is a hypothetical peptide sequence. Give the peptide fragments that will occur by enzymatic degradation using Trypsin and Chymotrypsin DARSKWKSENLIRTY 3) (10 pts) All superfamilies of serine proteases use the catalytic triad...
Which of the following would be considered the most acidic amino acid? alanine serine leucine glycine We were unable to transcribe this imageQuestion 3 2 pts When placed in water, proteins fold into discrete three dimensional shapes. Which amino acid is least likely to be found on the surface of a protein in water? lysine serine aspartic acid valine Describe how you would synthesize the dipeptide Leu-Gly. HTML Editor I EE xx, BIUA T T12pt
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IDIOSY CORE Nne Lecture test 2 on the chemicals of Lite Name: 51. Picture a globular protein in the cytoplasm of a cell. Where would you find the following amino acids: alanine and serine? COOCOO A Serine would be in the interior, and alanine would be on the exterior of the globular protein HN-C-H HŇ-Ć-H R Alanine would be in the Interior and serine would be on the exterior of the CHOH CH3 globular protein C Both serine and alanine...
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