Question

os 04 16. On a gas chromatograph the amount of each detected component is proportional to the relative peak area. How do you calculate this peak area? How do you determine the retention time? 03 M 02 0,1 10

Answer 1

gas phase, so Retention time (RT) is a measure of the time taken for a solute to, paus through a chromatography column. It is calculated as the time from injection to detection. The more soluble a compound in liq, phase , less time will be casried by the it has high netention time. tr = retention time for solute solute spent toA every point phase, but they spend diff erent time in of injee stationary point of phase ae according t=0 tr in Gc 2 same time at mobile time- tion their detection

interaction with stationary phase. if we take a un retained solute which do not stick into stationary phase and travel down with the same speed as the carrier gas. tm = time for all solvete spend in mobile phase (it is retention time of unretained solute to retention time time in stationary phase + time in mobile phase Ite=ts + tm S-stationary, phase mmobile phase. calculation of peak area : - The area directly proportional to amount of the compound. calculated taking the peak triangle Area of triangle of peak is Area can be approximately a = height of height of the peak + x width at half height