Question

Fluorescent Protein Gene Fusia for Subcellular Localization Imaging 5. (2 pts) You are studying the human alpha tubulin protein (which is a part of microtubules). You fuse the protein to a GFP in order to observe it. a. Why would you want to attach a GFP to tubulin (what kind of info would it show you) and why would you prefer this method to using a stain that binds to tubulin? 26 Humana EOFI Se Termine A Acid Figure 4 . Draw the construct of the GFP gene and the tubulin gene as you would fuse them (keep track of the N terminus and C erminus and how they fit with 5' and 3 of the DNA in the genes).

Answer 1

(5 o`Makcig a GEP fused tubulin would help located & Bidentify tabubin in a cell theseby faciitatingstudyting toe 3 7G localization, interadions pynamice. Vsing one from dtüdying the live dynbmics I tubulin mole cules. b). +4 STAKI poomoter 4FP (STOP c). 6 amino, acid linkes because They have to be in multiples 8 to avoid frame shifting. Alco, Š would hare be too short ohile >6 would be tov long consi dering the fact that éo GFP is within the protein sequence rhther them at the' ylanks oytos keletal elemento by polymerigation and D. Tubulin otgtlends - N-teiminal & c-tehinal shoild to free? hence both bend with thanner to "põeceeding & oucleding monomer. A GfP, at eilher ends would hinder this bonding.