Question

please answer #2 only

2- Anaerobic Jar OBSERVATIONS AND INTERPRETATIONS Indicate the amount of growth of each organism on each plate. no growth "3" = dense growth and "2" - degrees of growth in between Growth on Growth on Aerobic Plate Anaerobic Plate Organism Aerotolerance Category Al caligenes faecalis saerobes C. Sporagenes 3 S. anaerobes S. epidermidis 3 3 facultativer anderob P. Plbores ces 5. aerobes QUESTIONS Suppose that after incubation you examine the jar before opening it and see that the methylene blue strip is blue (or the resazurin strip is pink). a. What would you guess the internal environment to be aerobic or anaerobic? b. How would you expect the growth on the plates inside the jar to differ from the plates incubated outside the jar? Jar anaerobic growth incubation plate both

Answer 1

2) C. sporagenes would be most affected by the conditions described previously. C sopragenes is a strict anaerobe i.e., it can not survive in presence of oxygen. In the jar, methylene blue strip is used to check whether anaerobic condition is achieved or not. When methylene blue strip turns colorless, it is concluded that anaerobic conditions have been achieved. In the given condition - question 1, methylene blue strip is remained blue after incubation i.e, there was presence of oxygen in the jar which will not allow growth of C. sporagenes.