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Clinical Case: The Case of the Deadly Delay 5 of 11> Part F Sometimes, an ELISA (enzyme-linked immunosorbent assay) is used to detect anti-measles antibodies in a patient's serum. What type of ELISA could be used for this application? Select all that apply View Available Hint(s) Direct ELISA Sandwich ELISA ■ Indirect ELISA Agglutination ELISA Submit Part G

Answer 1

Elisa is based upon the estimation of specific antigen or antibodies present in the given serum sample, with the aid of conjugated enzymatic degradation of the substrate added in to the system. Measles is a contagious disease developed upon the infection caused by Morbilivirus. Epidemically. It is prevalent in developing countries with majority of the complications arising in infants and children below the age of 11 years. Dry cough, fever and characteristic development of rashes all over the body are the striking indications of measles. The technique of ELISA is applied to diagnose the given serum samples for the presence of measles IgM antibodies. Initially, the ELISA wells are coated with measles antigen, diluted with carbonated buffer. 100 ul of virus with 0.05M carbonated buffer is the initial coating mixture. Later the plates or wells are incubated overnight at 4°C. 300 ul of blocking buffer is added to the incubated mixture. To this mixture 100 ul of diluted serum is added. After an hour, the wells are washed with 300 ul. washing buffer. The well re coated again with 100 ul of anti-IgG conjugate and incubated for 30 min at room temperature. Later 100 pl of substrate tetramethylbenzidine is added to the wells and left in dark for 15 min. Then, the color developed or the absorbance of the ELISA well or plate is measured at 450 nm within 30 min of stop solution addition. It is indirect ELISA technique that is employed in the diagnosis of measles. Hence, option is correct and best justifies the statement.