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The starting concentration of the plasmid DNA is 100 ngul and you added 2μ1 to your cells. The cells were diluted to one ml prior to plating. First, determine how many transformants you would have if you plated the entire 1 ml of cell:s. Remember, you only plated half or your transformation reaction on plates 1 and 2. Add the number of transformants on plates 1 and 2, and then multiply by 2 for the total number transformants. Record that number here. You recorded this above on the previous page. 1,008 Second, convert ng of plasmid added to the transformation to μg. Record that number below and show your work. Finally, do a little division to calculate number of transformants obtained per μg of plasmid. Report your efficiency as # of transformants per ug (or TFsug). Show your work and dont forget your units.
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Answer #1

you havent provide information about cells.

if the transformation is successful and you plated all the culture then you'll get a lawn of cells. and if it is unsuccessful you'll get nothing on the plate.

1000 ng = 1ug.

conc = 100ng/ul. you added 2ul means 200ng of DNA means 0.2 ug of DNA.

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