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Question 25 (4 points) Using the provided information, calculate the original cell culture density. Show your...
J. Uvelle Unuwing data, calculate the original cell density of the culture tested. SHOW ALL WORK....
J. Uvelle Unuwing data, calculate the original cell density of the culture tested. SHOW ALL WORK. Plate 1 (105): 6421 colonies Plate 2 (106): 639 colonies Plate 3 (107): 68 colonies Plate 4 (108): 7 colonies 4. If you take lul of a 102 dilution and add it to 9.999ml H,O, what is the final DF? Show work. Experimental: Perform the serial dilution as seen below (9.9ml tubes=yellow top, 9.0 ml=blue) 1ml A 1ml A 1 ml A 100ul a...
2a. (4 pts) A potent mutagen was added to an E. coli culture growing in rich...
2a. (4 pts) A potent mutagen was added to an E. coli culture growing in rich broth. Prior experiments established that this F cell line, designated WWU113, could not synthesize leucine. You are interested in measuring the rate of induced reverse mutation. You plan to grow a liquid culture of the leu strain and then plate various dilutions of the culture onto selective media. What media should you use to grow up the liquid culture? This will be Media A...
1. Calculate the serial dilutions using the following diagrams. 1mL 25mL 5mL 5mL 99mL 75mL 45mL 5...
1. Calculate the serial dilutions using the following diagrams. 1mL 25mL 5mL 5mL 99mL 75mL 45mL 5mL Dilution: 0.1mL .01mL 0.1mL 1.0mL 1.9mL 9.99m 99.9mL 2mL Dilution: - when transferring 0.1mL from the corresponding tube? Show all your work in scientifie notation. Hint: Observe the diagram carefully 2.. Caleulate the dilutions for each tube; then calculate the dilutions for the four plates 1 mL or 1g sample 1 mL 1mL 1mL 1mL 1mL 1mL 1 mL ml 99 mL 0.1mL...
In order to count cells, the culture must be duted The technique o serialul precise and...
In order to count cells, the culture must be duted The technique o serialul precise and quantitative method alle e n al b e performed allow you to determine how many well per miller were the time the were made in overview knowvolume of culturedded to know Volume 0 sterile diluent aliquid that will not harm the cell The liquid in this tube is mixed and known volume of this culture is transferred to another known volume of sterile diluent...
J. Uvelle Unuwing data, calculate the original cell density of the culture tested. SHOW ALL WORK. Plate 1 (105): 6421 colonies Plate 2 (106): 639 colonies Plate 3 (107): 68 colonies Plate 4 (108): 7 colonies 4. If you take lul of a 102 dilution and add it to 9.999ml H,O, what is the final DF? Show work. Experimental: Perform the serial dilution as seen below (9.9ml tubes=yellow top, 9.0 ml=blue) 1ml A 1ml A 1 ml A 100ul a...
2a. (4 pts) A potent mutagen was added to an E. coli culture growing in rich broth. Prior experiments established that this F cell line, designated WWU113, could not synthesize leucine. You are interested in measuring the rate of induced reverse mutation. You plan to grow a liquid culture of the leu strain and then plate various dilutions of the culture onto selective media. What media should you use to grow up the liquid culture? This will be Media A...
1. Calculate the serial dilutions using the following diagrams. 1mL 25mL 5mL 5mL 99mL 75mL 45mL 5mL Dilution: 0.1mL .01mL 0.1mL 1.0mL 1.9mL 9.99m 99.9mL 2mL Dilution: - when transferring 0.1mL from the corresponding tube? Show all your work in scientifie notation. Hint: Observe the diagram carefully 2.. Caleulate the dilutions for each tube; then calculate the dilutions for the four plates 1 mL or 1g sample 1 mL 1mL 1mL 1mL 1mL 1mL 1 mL ml 99 mL 0.1mL...
In order to count cells, the culture must be duted The technique o serialul precise and quantitative method alle e n al b e performed allow you to determine how many well per miller were the time the were made in overview knowvolume of culturedded to know Volume 0 sterile diluent aliquid that will not harm the cell The liquid in this tube is mixed and known volume of this culture is transferred to another known volume of sterile diluent...
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