Question

HBD HBB GFP br or + bnl On GFP end of class! • Assignment: Researchers created a CRISPR/Cas9 construct targeting HBB, which e

0 0
Add a comment Improve this question Transcribed image text
Answer #1

Answer 1:

Here we are studying the ability of CRISPR/Cas9 nucleases to cause off-target cleavage. That's why these are DNA sequences being run on PCR.  

Answer 2:

The results show that there were mismatch mutations in HBB gene and cleavage occurred in HBB genes that is why different bands are formed in each lane, i.e. 1\mug, 2 \mug, 3 \mug and 4 \mug. Whereas, there is no cleavage seen in HBD gene, leading to one band formation in each lane.

Answer 3:

If Cas9 was not added, then single bands would have formed for HBB gene in all four lanes. The results for HBB and HBD would look similar.  

Add a comment
Know the answer?
Add Answer to:
HBD HBB GFP br or + bnl On GFP end of class! • Assignment: Researchers created...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions
ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT