Question

13 unread)-nicky Resources | Marietta Jump to... Prostaglandins are a class of eicosanoids, fatty acid derivatives with a variety of extremely potent actions on vertebrate tissues. They are responsible for Prostaglandins are derived from the 20-carbon enzyme prostaglandin endoperoxide synthase. This enzyme, a cyclooxygenase, uses oxygen to conver arachidonic acid to PGG2, the immediate precursor of many different prostaglandins. fever and inflammation and its associated pain. arachidonic acid in a reaction catalyzed by t (a) The kinetic data given in the table are for the reaction catalyzed by a mutant of prostaglandin endoperoxide synthase. Focusing here on the first two columns, determine the Vmex and Km of the enzyme. Rate of formation Arachidonic acid (mM) Rate of formationf PGG2 of PGG2 with 10 mg/ml 0215 0.258 0.387 0.645 1.51 17.7 19.9 25.1 31.8 41.3 11.0 12.7 17.0 23.4 34.4 Number mM/min Number mM Please socroll down (b) Ibuprofen is an inhibitor of prostaglandin endoperoxide synthase. By inhibiting the synthesis of prostaglandins, ibuprofen reduces inflammation and pain. Using the data in the first and third columns of the table, determine the type of inhibition that ibuprofen exerts on prostaglandin Previous ® Check Answer Next Et

Answer 1

We have a column of data which should be satisfying the Michaelis Menten equation. So here we have 2 unknowns in the equation and ample data to form the corresponding equations.

We can take any row as our data. For the below calculation 1st and 2 and row have been taken into consideration.

i.e