Question

biochemistry

Prostaglandins are a class of eicosanoids, fatty acid derivatives with a variety of extremely potent actions on vertebrate tissues. They are responsible for producing fever and inflammation and its associated pain. Prostaglandins are derived from the 20-carbon fatty acid arachidonic acid in a reaction catalyzed by the enzyme prostaglandin endoperoxide synthase. This enzyme, a cyclooxygenase, uses oxygen to convert arachidonic acid to PGG2, the immediate precursor of many different prostaglandins. Arachidonic Rate of formation Rate of formation of PGG, with 10 mg/ml acid (MM) of PGG2 (mM/min) ibuprofen (mM/min) 0.155 21.3 13.3 0.186 24.0 0.279 30.3 20.6 0.465 28.2 1.09 49.8 41.5 The kinetic data given in the table are for the reaction catalyzed by a mutant of prostaglandin endoperoxide synthase. Focusing here on the first two columns, determine the V... and K., of the enzyme. 15.4 VL Κ .- Ibuprofen is an inhibitor of prostaglandin endoperoxide synthase. By inhibiting the synthesis of prostaglandins, ibuprofen reduces inflammation and pain. Using the data in the first and third columns of the table, determine the type of inhibition that ibuprofen exerts on prostaglandin endoperoxide synthase. O uncompetitive inhibition O competitive inhibition O mixed inhibition

there is no really additional information besides the table and the graph used to slove the problem

Answer 1

Ymax = 49.8. [yelocity cwhere all of ... | the enzame is bound to substrat... 49,8 mliyinin km = (st at umarta [stat 44.4 | 10.19 min Ī -Ft Rau of Seachon for Non-competitive inhibition Vima Ukm als Vorge chose out of os o$5 cups of oths of ots of 95,359 .

(1) Vmax = 49,8 mm/min Velocity at which all of the enzyme is bound to. subs.toate km = [s] at Yaymax [s) at 24,9 milimin neo 19 mm. (2) Ibuprofen is a non- competitive inhibitor for the enzyme i It gives different may but ** same Km value.