The five proteins listed below were run on a cation exchange column at pH = 7...
The five proteins listed below were run on a cation exchange column at pH = 7
Cytochrome C pl = 10.7
Ribonuclease A pl = 9.5
Myoglobin pl = 7.4
Carbonic anhydrase pl = 6.0
Glucose oxidase pl = 4.2
Which of the following statements is TRUE?
A) Glucose oxidase and carbonic anhydrase will be separated in the low salt wash
b) Cytochrome C binds and then elutes first when the salt concentration is increased
c) decreasing the pH will elute the bound proteins from the column
d) ribonuclease A elutes after myoglobin when the salt concentration is increased
e) Glucose oxidase and carbonic anhydrase will bind to the column and elute at higher salt
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The five proteins listed below were run on a cation exchange
column at pH = 7...
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biochemistry
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#2 is Q HiTrap column where it is anion exchange column not
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2. Predict the order that the following proteins will elute from a CM Sephadex column run at pH 6.5 using a NaCI gradient: fibrinogen (pl-5.8), hemogloblin (pl7.1), ribonuclease A (pl7.8), lysozyme (pl-11), pepsin (pl-1.0) 3. In the laboratory we ran an immunoblot on fractions obtained from the alkaline phosphatase experiment. This procedure requires a number of steps, two different antibodies and a detection method. You should be...
need only part a and C. please and thank you
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