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Question 1. You are studying three mutations in yeast. The first mutation causes the Trp phenotype causes the His- phenotype of being unable to grow without histidine in the medium. The third mutation causes a small colony phenotype. Wild-type yeast are Trp+ His+ and big. You mate a Trp- haploid mutant yeast to a His-small haploid mutant yeast. You induce sporulation of the resulting diploid, and obtain the following tetrad types. (The number of tetrads of each type that you get (out of a total of 100) are shown after the tetrad type) of being unable to grow without tryptophan in the medium. The second mutation Tetrad Type A (15) Trp- Hist big; Trp-His+ small: Trp+ His-big; Trp+ His-small Tetrad Type B (80) Trp His+ big Trp- His+ big; Trp+ His- small; Trp+ His-small Tetrad Type C (5) Trp+ His-big Trp- His+ small; Trp-His-big: Trp+ His+ small A) Which Tetrad types are TTs, NPDs, and PD with reference to His and size genes. What is the genetic distance between them? B) Which Tetrad types are TTs, NPDs, and PD with reference to Trp and size genes. What is the genetic distance between them? C) Which Tetrad Types are TTs, NPDs, and PD with reference to Trp and His genes. What is the genetic distance between them? D) Draw a genetic map showing the correct relative order of the Trp, His, and size loci Q.2 Why is genetic map distance estimated from random spores generally lower than genetile map distance from tetrads.

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