Describe and explain a hypothetical microarray experiment used to compare transcription between control and estrogen-treated hepatocytes...
Describe and explain a hypothetical microarray experiment used to compare transcription between control and estrogen-treated hepatocytes (liver cells). Assume there at 10 genes on the microarray, and that the mRNA from control cells is labeled with a red fluorophore and the mRNA from the estrogen-treated cells is labeled with a green fluorophore. Please answer the question in complete, clear sentences. Explain how you could tell if the expression of a gene was:
a. the same for both samples.
b. higher in estrogen-treated cells
c. lower in estrogen-treated cells.
d. expression absent/undetectable in either cell type.
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Describe and explain a hypothetical microarray experiment used
to compare transcription between control and estrogen-treated
hepatocytes...
In female mice, both the glucocorticoid (cortisol) receptor and the estrogen receptor are expressed in liver...
In female mice, both the glucocorticoid (cortisol) receptor and the estrogen receptor are expressed in liver cells (hepatocytes). The control element to which the glucocorticoid receptor binds is a short-strip of DNA that can accommodate the binding of the glucocorticoid receptor dimer. This control element is called a glucocorticoid response element (GRE). It is located1000 bp downstream from the poly-A site for the PEPCK gene. When this gene is activated by cortisol, it helps the hepatocyte to release glucose into...
You are conducting a microarray experiment with yeast cells and have labeled the cDNAs generated from...
You are conducting a microarray experiment with yeast cells and have labeled the cDNAs generated from cells grown under normal condition (N) with a red dye and the cDNAs generated from the same cell line after the cells were subjected to a stress (S) with a green dye. For gene spot 15, you observe that the color was red, what does that mean? A. The number of chromosomes per cell with gene 15 was higher after stress (S) B. The...
can someone explain how to answer this with reasons why 30-45 mins after estrogen addition: Acchyiase...
can someone explain how to answer this with reasons why
30-45 mins after estrogen addition: Acchyiase (HAT, odde auhye 60-90 mins after estrogen addition: Reauitment ef Poy to open 7. T identify the Gene X DNA element responsible for regulation by a Growth Fagor, you perform a linker scanning experiment in which you mutate 10 bp Tegions centered around positions -205 to-5 in the Gene X promoter/promoter- proximal region (wt wild-type promoter). The promoter variants were tested for their ability...
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90170021 201901/P53 pd 8. (3) Lator you find out that you have access to a microarray for 10 gones that are associated with John's type of cancer. The microarray was done comparing normal tissue the microarray are shown below. One of the patients is John sequence of proteins. What are these mutations? How do the mutations change the 9. (2) If you were to suggest gene therapy to the family. how you describe what the main goal of this therapy...
You are conducting a microarray experiment with yeast cells and have labeled the cDNAs generated from cells grown under normal condition (N) with a red dye and the cDNAs generated from the same cell line after the cells were subjected to a stress (S) with a green dye. For gene spot 15, you observe that the color was red, what does that mean? A. The number of chromosomes per cell with gene 15 was higher after stress (S) B. The...
can someone explain how to answer this with reasons why
30-45 mins after estrogen addition: Acchyiase (HAT, odde auhye 60-90 mins after estrogen addition: Reauitment ef Poy to open 7. T identify the Gene X DNA element responsible for regulation by a Growth Fagor, you perform a linker scanning experiment in which you mutate 10 bp Tegions centered around positions -205 to-5 in the Gene X promoter/promoter- proximal region (wt wild-type promoter). The promoter variants were tested for their ability...
90170021 201901/P53 pd 8. (3) Lator you find out that you have access to a microarray for 10 gones that are associated with John's type of cancer. The microarray was done comparing normal tissue the microarray are shown below. One of the patients is John sequence of proteins. What are these mutations? How do the mutations change the 9. (2) If you were to suggest gene therapy to the family. how you describe what the main goal of this therapy...
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