Homework Answers
9, Except Option D, all the methods involve hybridization.
10. Option B, because the components of the cell have different densities, so they get separated accordingly.
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You are conducting a microarray experiment with yeast cells and have labeled the cDNAs generated from...
Describe and explain a hypothetical microarray experiment used to compare transcription between control and estrogen-treated hepatocytes...
Describe and explain a hypothetical microarray experiment used to compare transcription between control and estrogen-treated hepatocytes (liver cells). Assume there at 10 genes on the microarray, and that the mRNA from control cells is labeled with a red fluorophore and the mRNA from the estrogen-treated cells is labeled with a green fluorophore. Please answer the question in complete, clear sentences. Explain how you could tell if the expression of a gene was: a. the same for both samples. b. higher...
In DNA sequencing, ddNTPs differ from dNTPs in that ddNTPs are lacking a OH at the...
In DNA sequencing, ddNTPs differ from dNTPs in that ddNTPs are
lacking a OH at the
a.
2' carbon
b.
5' carbon
c.
3' carbon
please answer as many as you can!! I am low on questions and
could use the help!
Mother || Child II Male 1 Male 2 Results from a paternity test using DNA fingerprinting is shown. DNA was isolated from a mother, her child and 2 potential fathers. Primers designed to amplify different satellite DNA regions...
please help table 1 , Question 1 and 10 procedure is done which is the color...
please help
table 1 , Question 1 and 10
procedure is done which is the color result
taly Color in results from the slide microarray or attach a photo Label each gene appropriately O O o ооо Figure 1. Color Intensity chart Expression Ratios 16 1/4 1/8 1/16 Table 1 Gene 1 Gene 2 Gene 3 Gene 4 Gene 5 Gene 6 Expression Ratio Decimal Value Log2 Value Paper Microarray Exercise 1. Describe which genes were induced or repressed in...
Please help 15. During our paper lab experiment, we compared the tumor cells from one patient...
Please help
15. During our paper lab experiment, we compared the tumor cells from one patient to the normal cells of the same patient. If we wanted to do a larger study to give a larger picture of what is going on in colon cancer cells which of the following would be a better way to set up the experiment? A. Collect cancer cells and normal cells from 1000 patients and run 1000 different microarrays (one for each person) and...
Question 36 1 pts 36. You have a population of mammalian cells growing in media containing...
Question 36 1 pts 36. You have a population of mammalian cells growing in media containing enough growth factors. Which of the following graphs corresponds to these cells when their DNA content is analyzed 24 hours after they are induced to express high levels of a mutant form of Rb protein that cannot be phosphorylated? (Note: this cell line has its cell cycle around 22 hours) Control cells #1 #2 #3 #4 #5 #6 Cell number JAN ul. 2 2...
Question 10-12 10. During the mating process, yeast cells respond to pheromones secreted by other yeast...
Question 10-12
10. During the mating process, yeast cells respond to pheromones secreted by other yeast cells. These pheromones bind GPCRs on the surface of the responding cell and lead to the activation of proteins inside the cell. When a wild-type yeast cell senses the pheromone, its physiology changes in preparation for mating: the cell stops growing until it finds a mating partner. If yeast cells do not undergo the appropriate response after sensing a pheromone, they are considered sterile....
n a reproduction of an experiment famously performed by Louis Pasteur, you cultured yeast in the...
n a reproduction of an experiment famously performed by Louis Pasteur, you cultured yeast in the absence of oxygen (anaerobic growth) and measured (1) the rate of cell division, (2) the rate of glucose consumption, and (3) the intracellular ATP:ADP ratio. You then moved the cells to an incubator containing normal atmosphere (i.e., 21% oxygen), completed a second set of measurements, and made the following observations: The rate of cell division increased considerably after the addition of oxygen (aerobic growth)...
5. Sickle-cell disease is an interesting genetic disease. Normal homozygous individuals (SS) have normal blood cells...
5. Sickle-cell disease is an interesting genetic disease. Normal homozygous individuals (SS) have normal blood cells that are easily infected with the malarial parasite. Thus, many of these individuals become very ill from the parasite and many die. Individuals homozygous for the sickle-cell trait (ss) have red blood cells that readily collapse when deoxygenated. Although malaria cannot grow in these red blood cells, individuals often die because of the genetic defect. However, individuals with the heterozygous condition (Ss) have some...
You are given a flask with liquid media containing a population of S. pombe cells that...
You are given a flask with liquid media containing a population of S. pombe cells that have a temperature sensitive mutation of a gene that causes complete loss of function of the gene product at the restrictive temperature. The flask had been incubating at the permissive temperature before it was given to you, and the cells in the flask were replicating asynchronously with respect to the stage of the cell cycle. The mutated gene in this strain of yeast encodes...
90170021 201901/P53 pd 8. (3) Lator you find out that you have access to a microarray...
90170021 201901/P53 pd 8. (3) Lator you find out that you have access to a microarray for 10 gones that are associated with John's type of cancer. The microarray was done comparing normal tissue the microarray are shown below. One of the patients is John sequence of proteins. What are these mutations? How do the mutations change the 9. (2) If you were to suggest gene therapy to the family. how you describe what the main goal of this therapy...
In DNA sequencing, ddNTPs differ from dNTPs in that ddNTPs are
lacking a OH at the
a.
2' carbon
b.
5' carbon
c.
3' carbon
please answer as many as you can!! I am low on questions and
could use the help!
Mother || Child II Male 1 Male 2 Results from a paternity test using DNA fingerprinting is shown. DNA was isolated from a mother, her child and 2 potential fathers. Primers designed to amplify different satellite DNA regions...
please help
table 1 , Question 1 and 10
procedure is done which is the color result
taly Color in results from the slide microarray or attach a photo Label each gene appropriately O O o ооо Figure 1. Color Intensity chart Expression Ratios 16 1/4 1/8 1/16 Table 1 Gene 1 Gene 2 Gene 3 Gene 4 Gene 5 Gene 6 Expression Ratio Decimal Value Log2 Value Paper Microarray Exercise 1. Describe which genes were induced or repressed in...
Please help
15. During our paper lab experiment, we compared the tumor cells from one patient to the normal cells of the same patient. If we wanted to do a larger study to give a larger picture of what is going on in colon cancer cells which of the following would be a better way to set up the experiment? A. Collect cancer cells and normal cells from 1000 patients and run 1000 different microarrays (one for each person) and...
Question 36 1 pts 36. You have a population of mammalian cells growing in media containing enough growth factors. Which of the following graphs corresponds to these cells when their DNA content is analyzed 24 hours after they are induced to express high levels of a mutant form of Rb protein that cannot be phosphorylated? (Note: this cell line has its cell cycle around 22 hours) Control cells #1 #2 #3 #4 #5 #6 Cell number JAN ul. 2 2...
Question 10-12
10. During the mating process, yeast cells respond to pheromones secreted by other yeast cells. These pheromones bind GPCRs on the surface of the responding cell and lead to the activation of proteins inside the cell. When a wild-type yeast cell senses the pheromone, its physiology changes in preparation for mating: the cell stops growing until it finds a mating partner. If yeast cells do not undergo the appropriate response after sensing a pheromone, they are considered sterile....
You are given a flask with liquid media containing a population of S. pombe cells that have a temperature sensitive mutation of a gene that causes complete loss of function of the gene product at the restrictive temperature. The flask had been incubating at the permissive temperature before it was given to you, and the cells in the flask were replicating asynchronously with respect to the stage of the cell cycle. The mutated gene in this strain of yeast encodes...
90170021 201901/P53 pd 8. (3) Lator you find out that you have access to a microarray for 10 gones that are associated with John's type of cancer. The microarray was done comparing normal tissue the microarray are shown below. One of the patients is John sequence of proteins. What are these mutations? How do the mutations change the 9. (2) If you were to suggest gene therapy to the family. how you describe what the main goal of this therapy...
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