Question

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taly Color in results from the slide microarray or attach a photo Label each gene appropriately O O o ооо Figure 1. Color Intensity chart Expression Ratios 16 1/4 1/8 1/16 Table 1 Gene 1 Gene 2 Gene 3 Gene 4 Gene 5 Gene 6 Expression Ratio Decimal Value Log2 Value

table 1 , Question 1 and 10

procedure is done which is the color result

Answer 1

1. Genes 2, 3 and 4 are repressed in the lung cancer as the color readout is neither purple nor red.

Gene 6 is expressed in both the cells as purple color is read from the cDNA.

3. The blue color is mostly seen in most of the published microarray experiments indicating that the expression of normal lungs is abundant when compared to cancer lung.

4. Gene 5 showed increased transcription in the lung cancer cells as the cDNA labeled with Cy5 is read.

5. Genes 1, 2, 3, 4 and 6 are repressed in lung cancer cells. This is evident by the absence of red color in 1, 2, 3, and 6 whereas in 4 grey or black color indicate the absence of expression in both normal and cancer lung cells.

6. From the slide microarray, it can be observed that gene 4 is not expressed in either of the cell types. This indicates the lack of expression of the gene at the mRNA level or the gene might have deleted at the DNA level.