Homework Answers
Lineweaver -Burk equation-
1/V=(Km/Vmax)*1/[S] +1/Vmax
where V=reaction velocity
km=michaelis-menten constant
Vmax=maximum reaction velocity
[S]=substrate concentration
for 1/V vs 1/[S] plot ,a linear graph is obtained with slope=Km/Vmax, intercept=1/Vmax
[as per the linear eqn y=mx+c with m=slope,c=intercept]
b) it is competitive inhibition ,as km has increases ,but Vmax is unaffected ,intersects at 1/Vmax point(y-intercept same)
c) from the plot 1 slope=km/Vmax=0.071
from plot 2,slope=km'/Vmax=0.103
1/Vmax=y-intercept=0.046
Vmax=1/0.046=21.739 mM/s
plot 1,km=0.071*Vmax=1.543 mM/s
plot 2,km'=0.103*Vmax=2.239 mM/s
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Then choose Select Data in the Design tab on...
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