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Sanger sequencing utilizes flourochrome tagged ddNTP's that O allows for polymerization and identification of base sequences....
Sanger sequencing allows the identification of the bases and their order in a target piece of...
Sanger sequencing allows the identification of the bases and their order in a target piece of DNA. This technique exploits the way DNA is transcribed by using a primer and allowing new DNA to be created from its 3' end. In this technique, DNA is manufactured in vitro, which means that enzymes and molecules are combined in a test tube to allow the reactions to occur. In the original technique, four tubes were prepared, each containing the same reactants, but...
Dideoxy NTPs (ddNTPs) are used in sequencing reaction to terminate DNA polymerization reaction at various chain...
Dideoxy NTPs (ddNTPs) are used in sequencing reaction to terminate DNA polymerization reaction at various chain lengths. Which OH group on the carbohydrate (ribose) molecule is deoxidized in ddNTP, thus causes DNA polymerization to stop? O OH group on 1' carbon is deoxidized to-H O OH group on 2' carbon is deoxidized to-H O OH group on 3' carbon is deoxidized to -H O OH group on 4' carbon is deoxidized to-H O OH group on 5' carbon is deoxidized...
Why do ddNTPs in Sanger sequencing serve as terminators of the polymerase reaction? O because they...
Why do ddNTPs in Sanger sequencing serve as terminators of the polymerase reaction? O because they have an extra oxygen in Carbon 3 and no peptide bond can occur with the following nucleotide. O because they lack an oxygen in Carbon 3 and no phosphodiester bond can occur with the following nucleotide. O because they lack an oxygen in Carbon 5 and the polymerase detects that as a termination signal Because they lack a phosphate group in Carbon 5 and...
please help with all three! For DNA synthesis to take place in a Sanger sequencing reaction,...
please help with all three!
For DNA synthesis to take place in a Sanger sequencing reaction, the DNA polymerase must catalyze a reaction between the 3'- the last nucleotide and the 5- of the next nucleotide to be added. hydroxyl group, phosphate group O phosphate group, hydroxyl group O hydroxyl group, hydroxyl group O phosphate group, phosphate group QUESTION 6 millions of individual DNA fragments are isolated and sequenced in parallel during each machine run. O traditional whole-genome sequencing O...
Question 7 estion 7 0.3125 points Suppose that in a Sanger sequencing reaction, sequences are readable...
Question 7 estion 7 0.3125 points Suppose that in a Sanger sequencing reaction, sequences are readable for a few dozen nucleotides but then become increasingly difficult to road. It is determined that the chains are terminated to readily. What is the most likely cause? a. Too much of the dNTPs b. Insufficient fluorescent dye Too much fluorescent dye d. Incorrect primer e. Insufficient dNTPs Question of 20 Moving to another question will save this response Question 13 Which statement comparing...
MAT243 Project #3 MAT 243: Discrete Math Structures Project 3 Sequencing Sequences of numbers are a...
MAT243 Project #3
MAT 243: Discrete Math Structures Project 3 Sequencing Sequences of numbers are a useful mathematical tool for exploring transitions and trends. Sequences of symbols create words, and sequences of words create languages, both Human and Computer. While sequences of nucleotides create DNA, which in turn create new sequences of nucleotides called RNA, which in turn creates sequences of amino acids called proteins, which in turn create, among other things, humans that create, among other things, computers. The...
IDENTIFICATION of an UNKNOWN ORGANIC COMPOUND PRE LAB QUESTIONS DATE NAME 1. Circle AND name four...
IDENTIFICATION of an UNKNOWN ORGANIC COMPOUND PRE LAB QUESTIONS DATE NAME 1. Circle AND name four different functional groups in the compound to the right Macrocyclic lactone ring н сн N сн но. Нас Cн, O HO -O -он CH3 Hyc-OH CH3 Cн HyC OCH OH CH CH3 "сна 2. You have an unknown containing five carbon atoms, has a pH of 6, yields a yellow solution having no precipitate in a 2,4-DNP test, and produces a Jell-O like blue-green...
The following diagram represents a replication bubble associated with DNA synthesis. Based on this diagram, select...
The following diagram represents a replication bubble associated
with DNA synthesis. Based on this diagram, select all of the
options below that are true.
1 | 2 ---- Quadrants 1 and 4 are associated with lagging strand synthesis Quadrants 1 and 4 are associated with leading strand synthesis Quadrants 1 and 2 are associated with lagging strand synthesis Quadrants 3 and 4 are associated with lagging strand synthesis Synthesis of both daughter strands is completely continuous Telomerase activity is needed...
A nucleophile resembles a Lewis base because both have electron pairs to donate. The more tightly...
A nucleophile resembles a Lewis base because both have electron pairs to donate. The more tightly held an electron lone pair, the more basic it is (see polarizability in this paragraph and Leaving Group Stability below). Nucleophiles tend to prefer to bond to carbon and bases to H. Charged atoms are more nucleophilic than uncharged atoms. Nucleophilicity is inhibited by crowding which decreases a nucleophile's strength, but bulkiness tends to make a base stronger. Increasing polarizability of electrons favors nucleophilicity....
Question 1 1 pts Why is it important for DNA to have complementary base pairing? O...
Question 1 1 pts Why is it important for DNA to have complementary base pairing? O Complementary base pairing allows base pairs to be packed in the most energetically favorable arrangement inside of the double helix structure. O Complementary base pairing will pair a purine with a purine, which are a similar width, thus they are able to hold the sugar-phosphate backbone an equal distance apart along the DNA molecule o Complementary base pairing is only important for maintaining the...
Sanger sequencing allows the identification of the bases and their order in a target piece of DNA. This technique exploits the way DNA is transcribed by using a primer and allowing new DNA to be created from its 3' end. In this technique, DNA is manufactured in vitro, which means that enzymes and molecules are combined in a test tube to allow the reactions to occur. In the original technique, four tubes were prepared, each containing the same reactants, but...
Dideoxy NTPs (ddNTPs) are used in sequencing reaction to terminate DNA polymerization reaction at various chain lengths. Which OH group on the carbohydrate (ribose) molecule is deoxidized in ddNTP, thus causes DNA polymerization to stop? O OH group on 1' carbon is deoxidized to-H O OH group on 2' carbon is deoxidized to-H O OH group on 3' carbon is deoxidized to -H O OH group on 4' carbon is deoxidized to-H O OH group on 5' carbon is deoxidized...
Why do ddNTPs in Sanger sequencing serve as terminators of the polymerase reaction? O because they have an extra oxygen in Carbon 3 and no peptide bond can occur with the following nucleotide. O because they lack an oxygen in Carbon 3 and no phosphodiester bond can occur with the following nucleotide. O because they lack an oxygen in Carbon 5 and the polymerase detects that as a termination signal Because they lack a phosphate group in Carbon 5 and...
please help with all three!
For DNA synthesis to take place in a Sanger sequencing reaction, the DNA polymerase must catalyze a reaction between the 3'- the last nucleotide and the 5- of the next nucleotide to be added. hydroxyl group, phosphate group O phosphate group, hydroxyl group O hydroxyl group, hydroxyl group O phosphate group, phosphate group QUESTION 6 millions of individual DNA fragments are isolated and sequenced in parallel during each machine run. O traditional whole-genome sequencing O...
Question 7 estion 7 0.3125 points Suppose that in a Sanger sequencing reaction, sequences are readable for a few dozen nucleotides but then become increasingly difficult to road. It is determined that the chains are terminated to readily. What is the most likely cause? a. Too much of the dNTPs b. Insufficient fluorescent dye Too much fluorescent dye d. Incorrect primer e. Insufficient dNTPs Question of 20 Moving to another question will save this response Question 13 Which statement comparing...
MAT243 Project #3
MAT 243: Discrete Math Structures Project 3 Sequencing Sequences of numbers are a useful mathematical tool for exploring transitions and trends. Sequences of symbols create words, and sequences of words create languages, both Human and Computer. While sequences of nucleotides create DNA, which in turn create new sequences of nucleotides called RNA, which in turn creates sequences of amino acids called proteins, which in turn create, among other things, humans that create, among other things, computers. The...
IDENTIFICATION of an UNKNOWN ORGANIC COMPOUND PRE LAB QUESTIONS DATE NAME 1. Circle AND name four different functional groups in the compound to the right Macrocyclic lactone ring н сн N сн но. Нас Cн, O HO -O -он CH3 Hyc-OH CH3 Cн HyC OCH OH CH CH3 "сна 2. You have an unknown containing five carbon atoms, has a pH of 6, yields a yellow solution having no precipitate in a 2,4-DNP test, and produces a Jell-O like blue-green...
The following diagram represents a replication bubble associated
with DNA synthesis. Based on this diagram, select all of the
options below that are true.
1 | 2 ---- Quadrants 1 and 4 are associated with lagging strand synthesis Quadrants 1 and 4 are associated with leading strand synthesis Quadrants 1 and 2 are associated with lagging strand synthesis Quadrants 3 and 4 are associated with lagging strand synthesis Synthesis of both daughter strands is completely continuous Telomerase activity is needed...
A nucleophile resembles a Lewis base because both have electron pairs to donate. The more tightly held an electron lone pair, the more basic it is (see polarizability in this paragraph and Leaving Group Stability below). Nucleophiles tend to prefer to bond to carbon and bases to H. Charged atoms are more nucleophilic than uncharged atoms. Nucleophilicity is inhibited by crowding which decreases a nucleophile's strength, but bulkiness tends to make a base stronger. Increasing polarizability of electrons favors nucleophilicity....
Question 1 1 pts Why is it important for DNA to have complementary base pairing? O Complementary base pairing allows base pairs to be packed in the most energetically favorable arrangement inside of the double helix structure. O Complementary base pairing will pair a purine with a purine, which are a similar width, thus they are able to hold the sugar-phosphate backbone an equal distance apart along the DNA molecule o Complementary base pairing is only important for maintaining the...
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