the purity of a DNA sample is usually measured by A260/A280 ratio.
A260/A280 ratio of 1.8 is usually accepted as pure for DNA.
A260/A280 ratio.of 2.0 is accepted as pure for RNA.
for a DNA sample, A260/A280 ratio higher than 1.8 indicates the presence of RNA.
this can be fixed by adding RNase to the purified DNA sample.
what does it mean if the purity of the unknown dna sample was measured 2.54. also...
A 2.54 gram sample of an unknown gas is found to occupy a volume of 1.43 L at a pressure of 989 mm Hg and a temperature of 40°C. The molecular weight of the unknown gas is g/mol. Assume ideal behavior.
What does it mean that mean of error ? Does it also mean that mean of variance is zero? What is the difference between population mean and sample mean?
help !!!
1. A student spots an unknown sample on a TLC plate and develops it in dichloromehane solbv Only one spot, for which the Ry value is 0.15, is observed. Does this indicate that the unknown material is a pure compound? What can be done to verify the purity of the sample?
1. A student spots an unknown sample on a TLC plate and develops it in dichloromehane solbv Only one spot, for which the Ry value is 0.15,...
I'm determining the % Purity of KHP in an unknown sample. I have done two titrations: one with pure KHP titrated against a NaOH 0.1M solution, and the other is an unknown purity of KHP also titrated against a NaOH 0.1M solution. I am having trouble finding the percent purity of KHP, my professor said to recalculate my results because mine were too high (47.5%). Please help!!! Unknown Titration Trial Weight Initial Volume (mL) Final Volume (mL) 1 1.2030 1.37...
3. The melting point of unknown A was measured to be 110-120°C and unknown B was measured to be 105-109°C. What statement would make the best conclusion about the identity and purity of the product? (4 pts) A. Unknown A range of 110-120°C is the pure substance. Unknown B range of 105-109°C contains impurities. B. Unknown B range of 105-109°C is the pure substance. Unknown A range of 110-120°C contains impurities. C. Both Unknown A and B are pure substances....
How DNA Is Copied 4. What does it mean that the two strands of DNA are complementary? 5. What is DNA replication?, 6. Using your notes, book, and this assignment, place the steps of DNA replication in the correct order. a. The enzyme DNA polymerase moves along the exposed strands and adds complementary nucleotides to each nucleotide in each existing strand. b. The DNA double helix breaks or unzips down the middle between the base pairs. C. A complementary strand...
Why is thin layer chromatography used to determine the purity of a sample when you could use an IR spectrum? How does TLC confirm the purity?
A sample from a Normal distribution with an unknown mean u and known variance o = 45 was taken with n=9 samples giving sample mean of y = 3.6. (a) Construct a Hypothesis test with significance level a = 0.05 to test whether the mean is equal to 0 or it is greater than 0. What can you conclude based on the outcome of the sample? (b) Calculate the power of this test if the true value of the mean...
If the sample mean and sample standard deviation are the same when doing a study with an unknown mean and standard deviation of the population. What effect does increasing the sample size have on The P value and the margin of error (assuming the same confidence level)? Also, what effect does increasing the sample size have on the width of the confidence interval (assuming the same confidence level)?
If your unknown compound partially dissolves in a weak base does that mean that your unknown is a weak acid? If so, how?