State what happens to DNA as the sodium chloride (NaCl) concentration is increased in a solution...
State what happens to DNA as the sodium chloride (NaCl) concentration is increased
in a solution or buffer. Further, explain the biochemical events that take place in order for the phenomenon to occur.
Homework Answers
High sodium chloride in buffer induces DNA breakage.
Buffer is used to solublize DNA while preventing it from degradation. Salt present in buffer neutralize the charge of DNA's sugar phosphate and makes the DNA less soluble in water. Sodium chloride ( NaCl ) can be used to adjust the pH of buffers. High NaCl induce breakage of DNA and is repaired when NaCl is lowered. The increasing concentration of NaCl in a solution , damages the DNA and eventually breakers it. The high NaCl also causes accumulation of double strand breaking of DNA in S and G2 phase of cell cycle and thus repairing will inhibited .
Cations sheild negatively charged phosphate and eliminate the repulsion force between 2 parallel strands of DNA at high concentration of salt during renaturation.
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State what happens to DNA as the sodium chloride (NaCl)
concentration is increased
in a solution...
C. Concentration of a Sodium Chloride Solution C.1 Mass of evaporating dish Volume of NaCl solution...
C. Concentration of a Sodium Chloride Solution C.1 Mass of evaporating dish Volume of NaCl solution Mass of dish and NaCl solution C.4 Mass of dish and dry Naci C.2 17.4362 g 10.0 ml 25.6962 21.0062 g Calculations (Instruction can be found on p.86) C. Mass of NaCl solution (g) C.6 Mass of the dry NaCl salt (g) C.7 Mass/mass percent (show calculations.) C.8 Mass/volume percent (show calculations) C.9 Moles of NaCl (show calculations.) C.10 Volume of sample in liters...
If an aqueous solution is 2.17% (w/v) in sodium chloride, NaCl , what is the osmolarity...
If an aqueous solution is 2.17% (w/v) in sodium chloride, NaCl , what is the osmolarity of the solution?
Find molarity of solution NaCl given ion concentration
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100μL of 1M sodium chloride (NaCl) is mixed with100μL of water into a single tube. What...
100μL of 1M sodium chloride (NaCl) is mixed with100μL of water into a single tube. What is the final concentration of sodium chloride?
Perform the following concentration conversions (c) "Normal saline" is an aqueous solution of sodium chloride that...
Perform the following concentration conversions (c) "Normal saline" is an aqueous solution of sodium chloride that is widely used for many medical purposes, manufactured as a sterile solution of 9 grams of NaCl per liter. Calculate the molarity of normal saline. (d) A solution is prepared of 30.0 g of sucrose dissolved in 300.0 mL of water. what is the concentration of this solution expressed as % sucrose by mass? (The density of water is 1.0 g/mL.)
(a) Saturated Sodium Chloride. Watch Video-3a where to 4 mL of saturated (5.4 M) NaCl in...
(a) Saturated Sodium Chloride. Watch Video-3a where to 4 mL of saturated (5.4 M) NaCl in a 13 x 100mm test tube, 2 mL of concentrated (12 M) HC) is added. Write down the chemical reaction(s) involved, being sure to include the state of matter of all reactants and products so it is clear if there is a solid/precipitate expected. (1 point) Calculate the total Cl concentration in the NaCl solution after adding 2 mL of 12 M HCl to...
What is the osmolarity of a 0.8% solution of sodium chloride (NaCl)? Atomic weights: (Na - 23, CI - 35.5)
What is the osmolarity of a 0.8% solution of sodium chloride (NaCl)? Atomic weights: (Na - 23, CI - 35.5)
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C. Concentration of a Sodium Chloride Solution C.1 Mass of evaporating dish Volume of NaCl solution Mass of dish and NaCl solution C.4 Mass of dish and dry Naci C.2 17.4362 g 10.0 ml 25.6962 21.0062 g Calculations (Instruction can be found on p.86) C. Mass of NaCl solution (g) C.6 Mass of the dry NaCl salt (g) C.7 Mass/mass percent (show calculations.) C.8 Mass/volume percent (show calculations) C.9 Moles of NaCl (show calculations.) C.10 Volume of sample in liters...
4. What is the mass % of NaCl if 15 g sodium chloride are dissolved in 374 g H2O? (Hint: don't forget that solution mass = solute + solvent mass.) 5. What mass of acetic acid (HC2H302 = 60 g/mol) must be dissolved in 1340 g H2O to produce a 2.75 m solution? 6. What is the new volume of a solution if 437 mL of a .968 M solution are diluted to a .315 M solution? 7. One solution...
(a) Saturated Sodium Chloride. Watch Video-3a where to 4 mL of saturated (5.4 M) NaCl in a 13 x 100mm test tube, 2 mL of concentrated (12 M) HC) is added. Write down the chemical reaction(s) involved, being sure to include the state of matter of all reactants and products so it is clear if there is a solid/precipitate expected. (1 point) Calculate the total Cl concentration in the NaCl solution after adding 2 mL of 12 M HCl to...
What is the osmolarity of a 0.8% solution of sodium chloride (NaCl)? Atomic weights: (Na - 23, CI - 35.5)
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