In order for chymotrypsin to function, His-57 must be deprotonated. At pH = 7, approximately what...
In order for chymotrypsin to function, His-57 must be deprotonated. At pH = 7, approximately what percentage of chymotrypsin proteins are active? Assume the pKa of His is 6.
| a. 24% |
| b. 0% |
| c. 9% |
| d. 91% |
| e. 100% |
| f. 76% |
Homework Answers
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In order for chymotrypsin to function, His-57 must be
deprotonated. At pH = 7, approximately what...
Biochem help 2 14. What would be the net charge on the dipeptide Ser-His at pH-...
Biochem help 2
14. What would be the net charge on the dipeptide Ser-His at pH- 6.04? (Choose the one best answer.) a) 1.5 b) +1; c) +0.5 d) 0 e) -0.5; 15. The pKa of a lysine side chain in a protein ending up on the outside of a globular protein has a different pKa than if the lysine is buried within the interior of a protein. What would be the expected pKa of a side chain of lysine...
Can someone explain how these work? 4. For a solution of Cys (C), at what pH...
Can someone explain how these work?
4. For a solution of Cys (C), at what pH would the net charge be closest to 1? a) 10.7 b) 8.4 c) (1.9-8.4)/2 = 5.5 d) (10.7 + 8.4)/2 = 9.55 5. For a solution of Tyr (Y), what percentage of the side chain R group's OH would be deprotonated at pH = 10.5? a) 0% b) 25% c) 50% d) 100% 6. For the peptide His -Asp-Gln-Glu-Ala- Lys (HDQEAK), what would be...
2. hypothesize the best conditions (pH and temperature) under which the enzyme chymotrypsin functions with an...
2. hypothesize the best conditions (pH and temperature) under
which the enzyme chymotrypsin functions with an appropriate
reference. also, hypothesize what will occur in the presence of an
inhibitor and the type of inhibition.
EXPERIMENT 5: ENZYME ACTIVITY WITH a-CHYMOTRYPSIN Prelab Assignment 1. Prepare a flow chart, covering one half of the experimental work (either part A and C if your lab bench is on the window side of the lab or part B and D if your locker is...
a. Which spot corresponds to the protein with the largest molecular weight? b. When pH = 7, what order would the follow...
a. Which spot corresponds to the protein with the largest
molecular weight?
b. When pH = 7, what order would the following peptides elute
from a column filled with cation exchange resin? Size exclusion
column?
Protein 1 – 400 kDa;
10%A 5%E, 5%F, 10% G, 5% H, 10%I, 10%K, 10%L 10%R, 5%P, 5%S, 10%W,
5%Y
Protein 2: 40 kDa
5%A, 5%C, 5%D, 5%E, 5%F, 10%G, 5%I, 10%K, 5%L, 5%M, 5%N, 10%P, 5%R,
5%S, 5%T, 5%V, 5%W,
Protein 3 – 175...
7. What is the pH of a sol a. 5.72 b. 4.27 c. e pH of...
7. What is the pH of a sol a. 5.72 b. 4.27 c. e pH of a solution containing 5.3 x 10% M hydroxide ion concentration? d.-4.27 e.-9.72 9.72 what is the pH of a solution containing 6 mM acetic acid (K, of acetic acid is 1.8 x 10) a. -0.77 d. 2.92 b. 1.98 e. 3.49 C. 2.22 9. One of the strongest acids known is called fluorosulfonic acid, HSO3F (pka --10). What the pOH of 1M of this...
Function f is graphed. y 9 8 7 6+ y = f(x) CT 4+ 3+ 2+...
Function f is graphed. y 9 8 7 6+ y = f(x) CT 4+ 3+ 2+ 1+ H4+ 29-8-7 -6 -5 -4 3-2 1 2 3 4 5 6 7 8 9 -2 -3 4 -50 1 A 2+ M من 4 -58 -6 -8 -9 What appears to be the value of lim f(x)? 20+ 07 0-5 Unbounded Function g is graphed. → see y= g(2) 9 8 7+ 6+ 5+ 4+ es 2+ 1+ A+++ -9 -8 -7...
x+2 7 21. multiple choice. Any iten may! y have more than one correct answer. 2....
x+2 7 21. multiple choice. Any iten may! y have more than one correct answer. 2. what is the domain of f(x) = x²-3x-28 (10 m) (-2,-4) u (-4,-2) +(-2,7) v (7,) b) (-2,-4) u (-4,7) 0 (7,-) c) (-0, -u) u 17, s ) d) (-2,-2) u (-2,-) e) (-4,7) 22, 22, which equation can be used to find the equation of a line that passes through (2,-5) and is perpendicular to 2x + 3y = 7? (1 pt)...
When the function f (x) is expanded in a power series about æ = 0, what will be the radius of con...
When the function f (x) is expanded in a power series about æ = 0, what will be the radius of convergence of the series. Pick the answer nearest to your result. a)0 b)I c)2 d) 3 e) 4 f)5 g)6 h) 7 i) 8 j)9
When the function f (x) is expanded in a power series about æ = 0, what will be the radius of convergence of the series. Pick the answer nearest to your result. a)0 b)I...
You are given a binary tree of the form: Each node in the tree has a...
You are given a binary tree of the form:
Each node in the tree has a left child and a right child. Each
of the children will be extended as a linked list. Every node has
the following attributes: key, left node, right node, and next
node. The next node allows a node, that is a part of the tree, to
be extended as a linked list. The diamonds represent the next
nodes, which are part of the linked list...
7. Identify the impulse response function for the differential equation below. y" +24' + 5y =...
7. Identify the impulse response function for the differential equation below. y" +24' + 5y = sin(t), y(0) = 1, y'(0) = 2. (a) Not enough information to tell (b) h(t) = 2e' sin(t) (c) h(t) = ecos(2t) (d) h(t) = {e- sin(21) 8. Which of the following equations is valid for functions f(t) and g(0)? (a) C{28 +7.9}(s) = 2C{S}(s) +tL{9}(3) (b) C{t.g}(8) = -(L{9}) (c) C{e-at.g}(s) = ({9}(s - a) (d) None of the above.
Biochem help 2
14. What would be the net charge on the dipeptide Ser-His at pH- 6.04? (Choose the one best answer.) a) 1.5 b) +1; c) +0.5 d) 0 e) -0.5; 15. The pKa of a lysine side chain in a protein ending up on the outside of a globular protein has a different pKa than if the lysine is buried within the interior of a protein. What would be the expected pKa of a side chain of lysine...
Can someone explain how these work?
4. For a solution of Cys (C), at what pH would the net charge be closest to 1? a) 10.7 b) 8.4 c) (1.9-8.4)/2 = 5.5 d) (10.7 + 8.4)/2 = 9.55 5. For a solution of Tyr (Y), what percentage of the side chain R group's OH would be deprotonated at pH = 10.5? a) 0% b) 25% c) 50% d) 100% 6. For the peptide His -Asp-Gln-Glu-Ala- Lys (HDQEAK), what would be...
2. hypothesize the best conditions (pH and temperature) under
which the enzyme chymotrypsin functions with an appropriate
reference. also, hypothesize what will occur in the presence of an
inhibitor and the type of inhibition.
EXPERIMENT 5: ENZYME ACTIVITY WITH a-CHYMOTRYPSIN Prelab Assignment 1. Prepare a flow chart, covering one half of the experimental work (either part A and C if your lab bench is on the window side of the lab or part B and D if your locker is...
a. Which spot corresponds to the protein with the largest
molecular weight?
b. When pH = 7, what order would the following peptides elute
from a column filled with cation exchange resin? Size exclusion
column?
Protein 1 – 400 kDa;
10%A 5%E, 5%F, 10% G, 5% H, 10%I, 10%K, 10%L 10%R, 5%P, 5%S, 10%W,
5%Y
Protein 2: 40 kDa
5%A, 5%C, 5%D, 5%E, 5%F, 10%G, 5%I, 10%K, 5%L, 5%M, 5%N, 10%P, 5%R,
5%S, 5%T, 5%V, 5%W,
Protein 3 – 175...
7. What is the pH of a sol a. 5.72 b. 4.27 c. e pH of a solution containing 5.3 x 10% M hydroxide ion concentration? d.-4.27 e.-9.72 9.72 what is the pH of a solution containing 6 mM acetic acid (K, of acetic acid is 1.8 x 10) a. -0.77 d. 2.92 b. 1.98 e. 3.49 C. 2.22 9. One of the strongest acids known is called fluorosulfonic acid, HSO3F (pka --10). What the pOH of 1M of this...
Function f is graphed. y 9 8 7 6+ y = f(x) CT 4+ 3+ 2+ 1+ H4+ 29-8-7 -6 -5 -4 3-2 1 2 3 4 5 6 7 8 9 -2 -3 4 -50 1 A 2+ M من 4 -58 -6 -8 -9 What appears to be the value of lim f(x)? 20+ 07 0-5 Unbounded Function g is graphed. → see y= g(2) 9 8 7+ 6+ 5+ 4+ es 2+ 1+ A+++ -9 -8 -7...
x+2 7 21. multiple choice. Any iten may! y have more than one correct answer. 2. what is the domain of f(x) = x²-3x-28 (10 m) (-2,-4) u (-4,-2) +(-2,7) v (7,) b) (-2,-4) u (-4,7) 0 (7,-) c) (-0, -u) u 17, s ) d) (-2,-2) u (-2,-) e) (-4,7) 22, 22, which equation can be used to find the equation of a line that passes through (2,-5) and is perpendicular to 2x + 3y = 7? (1 pt)...
When the function f (x) is expanded in a power series about æ = 0, what will be the radius of convergence of the series. Pick the answer nearest to your result. a)0 b)I c)2 d) 3 e) 4 f)5 g)6 h) 7 i) 8 j)9
When the function f (x) is expanded in a power series about æ = 0, what will be the radius of convergence of the series. Pick the answer nearest to your result. a)0 b)I...
You are given a binary tree of the form:
Each node in the tree has a left child and a right child. Each
of the children will be extended as a linked list. Every node has
the following attributes: key, left node, right node, and next
node. The next node allows a node, that is a part of the tree, to
be extended as a linked list. The diamonds represent the next
nodes, which are part of the linked list...
7. Identify the impulse response function for the differential equation below. y" +24' + 5y = sin(t), y(0) = 1, y'(0) = 2. (a) Not enough information to tell (b) h(t) = 2e' sin(t) (c) h(t) = ecos(2t) (d) h(t) = {e- sin(21) 8. Which of the following equations is valid for functions f(t) and g(0)? (a) C{28 +7.9}(s) = 2C{S}(s) +tL{9}(3) (b) C{t.g}(8) = -(L{9}) (c) C{e-at.g}(s) = ({9}(s - a) (d) None of the above.
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