Consider the data collected for an enzyme‑catalyzed reaction.
[?] (??)[S] (mM) | ?0 (??⋅?−1)v0 (mM⋅s−1) |
---|---|
0.200.20 | 0.860.86 |
0.330.33 | 1.201.20 |
2.002.00 | 2.422.42 |
4.004.00 | 2.692.69 |
Determine ?max and ?m for this reaction.
Consider the data collected for an enzyme‑catalyzed reaction. [?] (??)[S] (mM) ?0 (??⋅?−1)v0 (mM⋅s−1) 0.200.20 0.860.86...
Consider the data collected for an enzyme-catalyzed reaction [S] (mM) vo (mM s1 0.25 2.00 0.50 3.33 1.00 5.00 2.00 6.67 Determine Vmax and K,,m for this reaction. mM s- Vmax mM
Consider the data collected for an enzyme-catalyzed reaction. [S] (MM) 2 (MM :5-1) 0.20 0.86 0.33 1.20 2.00 2.42 4.00 2.69 Determine Vmax and Km for this reaction. mMs-1 Km = mM
Attempt 2 Question 19 of 41 Consider the data collected for an enzyme-catalyzed reaction. [S] (mM) 0.20 0.37 0.50 0.83 2.22 2.00 3.08 4.00 Determine Vmax and Km for this reaction 9.9999 mM s-1 Vmax 0.9999 Km mM =
Suppose that the following data are obtained for an enzyme-catalyzed reaction: [S] (mM) V (mmol ml-1min-1) 0.1 3.33 0.2 5.00 0.5 7.14 0.8 8.0 1.0 8.33 2.0 9.09 a.) From a double-reciprocal plot of the data, determine Km and Vmax. b.) Assuming that the enzyme present in the system had a concentration of 10-6 M, calculate its turnover number.
3.33 3. Suppose that the following data are obtained for an enzyme-catalyzed reaction: [S(mm) V (mmol ml-Imin-1) 0.1 0.2 5.00 7.14 8.0 1.0 8.33 9.09 (a) From a double-reciprocal plot of the data, determine Km and Vmax. (b) Assuming that the enzyme present in the system had a concentration of 10-6 M, calculate its turnover number 0.8 2.0
CHEM3250 Assignment-Enzyme Inhibition Consider the data below for an enzyme catalyzed reaction. The rate of the reaction has been determined with and without an inhibitor. A total concentration of enzyme of 20 uM was used in the experiment. SHOW WORK AND UNITS!!! Without Inhibitor With Inhibitor [substrate] (mM)Rate of formation of te of formation of product product (mM/min) mM/min) 6.67 5.25 0.49 7.04 38.91 1.0 2.2 6.9 41.8 44.0 1.5 3.5 1 a) On the same graph, plot the data...
For an enzyme catalyzed reaction, the following data were obtained. [S], M Rate, M-min-1 0.0143 0.005 0.010 0.025 0.050 0.075 0.0208 0.0294 0.0345 0.0360 Using these data, determine the Michaelis constant for this enzyme system.
4. The following data were obtained for an enzyme-catalyzed reaction. Determine whether the enzyme is an allosteric enzyme. (5pts) Submit graph [S] (M X 104) v (umoles/liter/min) 1 1 2 3 4 5 6 7 2 4 7 15 35 55 75 80 83 85 85 8 9 10 11 12
(15 points) Consider the following reaction: CO2+H20 F HCO; +H* This reaction is catalyzed by the enzyme carbonic anhydrase. The following data was obtained for an initial enzyme concentration of 2.3 nM and temperature of 0.5°C: [CO2] (MM) 1.25 Rate (M/s) 2.8x10-5 5.00x 10-5 8.33x10-5 1.67x10-4 2.5 5.0 20.0 Determine Km and Vmax for this reaction at the given enzyme concentration and temperature.
20.8 2) Consider the following data for an enzyme-catalyzed hydrolysis reaction in the presence and absence of inhibitor I using a Michaelis-Menten plot, determine Kn for both inhibited and uninhibited reactions. What kind of inhibition is this? [Substrate)(M) vo (umol/min) Vos (moles/min) 6x106 4.2 1x105 29 5.8 2x105 6x109 13.6 1.8x10* 16.2