4. The following data were obtained for an enzyme-catalyzed reaction. Determine whether the enzyme is an...
4. The following data were obtained for an enzyme-catalyzed reaction. Determine whether the enzyme obeys hyperbolic or sigmoidal kinetics. SUBMIT GRAPH [S] (MX 104) v (umoles/liter/min) V 6.25 12.5 25 50 100 200 400 800 1.54 5.88 20 50 80 94.12 98.46 99.611
3. The following data were obtained for an enzyme-catalyzed reaction. Determine whether the enzyme obeys hyperbolic or sigmoidal kinetics. [S] (M X 10) v (umoles/liter/min) 6.25 12.5 1.54 5.88 25 20 50 100 200 400 50 80 94.12 98.46 99.61 800
For an enzyme catalyzed reaction, the following data were obtained. [S], M Rate, M-min-1 0.0143 0.005 0.010 0.025 0.050 0.075 0.0208 0.0294 0.0345 0.0360 Using these data, determine the Michaelis constant for this enzyme system.
The following data were recorded for the enzyme-catalyzed reaction. Substrate concentration (M) 6.25 x 100 7.50 x 10 1.00 x 10-4 1.00 x 10-3 Reaction velocity (nM/min) 15 56 60 75 (1) Estimate Km and Vmax- (2) What would V be at S=2.5 x 10-5 ?
CHEM3250 Assignment-Enzyme Inhibition Consider the data below for an enzyme catalyzed reaction. The rate of the reaction has been determined with and without an inhibitor. A total concentration of enzyme of 20 uM was used in the experiment. SHOW WORK AND UNITS!!! Without Inhibitor With Inhibitor [substrate] (mM)Rate of formation of te of formation of product product (mM/min) mM/min) 6.67 5.25 0.49 7.04 38.91 1.0 2.2 6.9 41.8 44.0 1.5 3.5 1 a) On the same graph, plot the data...
5) (14 marks) The following kinetic data were obtained for an enzyme in the absence of inhibitor (1), and in the presence of an inhibitor at 5 mM concentration (2). Assume[ET] is the same in each experiment. [S] (MM) (1) v(umol/mL sec) 12 (2) v(umol/mL sec) 4.3 1 8 2 4 20 29 14 21 8 35 12 40 26 a. Using a graphing program (excel or sigmaplot) construct a lineweaver burke plot representing the uninhibited reaction and the inhibited...
4. The following data were obtained from an enzyme kinetics experiment. Graph the data using a Lineweaver-Burk plot and determine, by inspection of the graph, the values for Km and Vmax. ISI (M) V (nmol/min) 0.20 0.26 0.33 1.00 1.43 1.67 2.08 3.33 5. You measured the kinetics of an enzyme activity as a function of substrate concentration (see Table). The enzyme concentration was maintained constant at a level of 1 M. [S] AM Vopmol/min 2.9 3.8 4.4 Plot the...
3.33 3. Suppose that the following data are obtained for an enzyme-catalyzed reaction: [S(mm) V (mmol ml-Imin-1) 0.1 0.2 5.00 7.14 8.0 1.0 8.33 9.09 (a) From a double-reciprocal plot of the data, determine Km and Vmax. (b) Assuming that the enzyme present in the system had a concentration of 10-6 M, calculate its turnover number 0.8 2.0
Suppose that the following data are obtained for an enzyme-catalyzed reaction: [S] (mM) V (mmol ml-1min-1) 0.1 3.33 0.2 5.00 0.5 7.14 0.8 8.0 1.0 8.33 2.0 9.09 a.) From a double-reciprocal plot of the data, determine Km and Vmax. b.) Assuming that the enzyme present in the system had a concentration of 10-6 M, calculate its turnover number.
The following data has been obtained for an enzyme-catalyzed reaction with two different initial enzyme concentrations of 0.025g/L and 0.015g/L. V (g/L.min) [S] (g/L) Eo = 0.025g/L Ep = 0.015g/L 56.8 1.75 1.05 28.4 1.58 0.97 19.1 1.47 0.89 14.3 1.38 0.83 11.4 1.31 0.77 9.4 1.22 0.73 8.2 1.15 0.69 7.1 1.08 0.66 (a) Using the Lineweaver-Burke plot, determine Km and Vm for Eo = 0.025g/L (b) Using the Lineweaver-Burke plot, determine Km and Vm for Ep = 0.015g/L...