Question

4. The following data were obtained for an enzyme-catalyzed reaction. Determine whether the enzyme obeys hyperbolic or sigmoidal kinetics. SUBMIT GRAPH [S] (MX 104) v (umoles/liter/min) V 6.25 12.5 25 50 100 200 400 800 1.54 5.88 20 50 80 94.12 98.46 99.611

Answer 1

Solution: The kinetics observed by the enzyme in the reaction could be understood by plotting a graph between v(rate of reaction) and S(substrate concentration) and then analysing the shape of the graph whether its sigmoidal or hyperbolic.

The graph drawn for the above data is as follows:

Graph of v v/s S 120 98.46 99.61 94.12 100 80 80 v (micromoles/lit.min) 60 50 40 20 20 0 0 100 200 300 600 700 800 900 400 500 S(Mx1044)

Hence, it is clear that the enzyme follows hyperbolic kinetics.