Question

3. The following data were obtained for an enzyme-catalyzed reaction. Determine whether the enzyme obeys hyperbolic or sigmoidal kinetics. [S] (M X 10) v (umoles/liter/min) 6.25 12.5 1.54 5.88 25 20 50 100 200 400 50 80 94.12 98.46 99.61 800

Answer 1

When we plot the graph between the V(rate) vs [S] substrate concentration .

there are two types of kinetics are observed.

1. sigmoidal

2. hyperbolic.

if we plot the date given....

From the above graph ..we can see that this is hyperbolic type kinetics.

v (umol/liter/min) vs [S] (Mx10^4) Plot Area -V vs [S] 200 400 600 800 1000 [S]