![For an enzyme catalyzed reaction, the following data were obtained. [S], M Rate, M-min-1 0.0143 0.005 0.010 0.025 0.050 0.075](http://img.homeworklib.com/questions/494841f0-70e0-11ea-95b0-2db0cbc6236d.png?x-oss-process=image/resize,w_560)
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![Км for enzyme catalysis reaction, the rate expression is given by - [53. = Substrate concentration K₂[€] [53. [El. = [s]+ km](http://img.homeworklib.com/questions/49b57b60-70e0-11ea-a842-d9d08b812b90.png?x-oss-process=image/resize,w_560)
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For an enzyme catalyzed reaction, the following data were obtained. [S], M Rate, M-min-1 0.0143 0.005...
4. The following data were obtained for an enzyme-catalyzed reaction. Determine whether the enzyme is an...
4. The following data were obtained for an enzyme-catalyzed reaction. Determine whether the enzyme is an allosteric enzyme. (5pts) Submit graph [S] (M X 104) v (umoles/liter/min) 1 1 2 3 4 5 6 7 2 4 7 15 35 55 75 80 83 85 85 8 9 10 11 12
3. The following data were obtained for an enzyme-catalyzed reaction. Determine whether the enzyme obeys hyperbolic...
3. The following data were obtained for an enzyme-catalyzed reaction. Determine whether the enzyme obeys hyperbolic or sigmoidal kinetics. [S] (M X 10) v (umoles/liter/min) 6.25 12.5 1.54 5.88 25 20 50 100 200 400 50 80 94.12 98.46 99.61 800
4. The following data were obtained for an enzyme-catalyzed reaction. Determine whether the enzyme obeys hyperbolic...
4. The following data were obtained for an enzyme-catalyzed reaction. Determine whether the enzyme obeys hyperbolic or sigmoidal kinetics. SUBMIT GRAPH [S] (MX 104) v (umoles/liter/min) V 6.25 12.5 25 50 100 200 400 800 1.54 5.88 20 50 80 94.12 98.46 99.611
Under what circumstances does an enzyme catalyzed reaction rate resemble a non-enzyme catalyzed reaction? At very...
Under what circumstances does an enzyme catalyzed reaction rate resemble a non-enzyme catalyzed reaction? At very low concentrations of substrate (Km is greater than S) the Michaelis-Menton equation can be simplified to? At very high concentrations of substrate, the Michaelis-Menton equation can be simplified to? How do you determine the initial rate of reaction
Suppose that the following data are obtained for an enzyme-catalyzed reaction: [S] (mM) V (mmol ml-1min-1)...
Suppose that the following data are obtained for an enzyme-catalyzed reaction: [S] (mM) V (mmol ml-1min-1) 0.1 3.33 0.2 5.00 0.5 7.14 0.8 8.0 1.0 8.33 2.0 9.09 a.) From a double-reciprocal plot of the data, determine Km and Vmax. b.) Assuming that the enzyme present in the system had a concentration of 10-6 M, calculate its turnover number.
3.33 3. Suppose that the following data are obtained for an enzyme-catalyzed reaction: [S(mm) V (mmol...
3.33 3. Suppose that the following data are obtained for an enzyme-catalyzed reaction: [S(mm) V (mmol ml-Imin-1) 0.1 0.2 5.00 7.14 8.0 1.0 8.33 9.09 (a) From a double-reciprocal plot of the data, determine Km and Vmax. (b) Assuming that the enzyme present in the system had a concentration of 10-6 M, calculate its turnover number 0.8 2.0
20.8 2) Consider the following data for an enzyme-catalyzed hydrolysis reaction in the presence and absence...
20.8 2) Consider the following data for an enzyme-catalyzed hydrolysis reaction in the presence and absence of inhibitor I using a Michaelis-Menten plot, determine Kn for both inhibited and uninhibited reactions. What kind of inhibition is this? [Substrate)(M) vo (umol/min) Vos (moles/min) 6x106 4.2 1x105 29 5.8 2x105 6x109 13.6 1.8x10* 16.2
The reaction, A+B+C Products, was studied kinetically and the following data were obtained. d[A] 110M- 7.5...
The reaction, A+B+C Products, was studied kinetically and the following data were obtained. d[A] 110M- 7.5 [A). [B] [C]. dt 0.010 0.005 0.010 5 0.010 0.005 0.015 0.010 0.015 0.010 45 0.020_0.005_0010 14.1 A. Determine the integrated rate law for the reaction. B. Determine the rate constant.
Enzyme Kinetics Problem The initial rate for an enzyme-catalyzed reaction has been determined at a number...
Enzyme Kinetics Problem The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate concentrations. Data are given below: 5 27 23 65 1. Estimate V and K from a Michaelis-Menten graph of V versus [S] 2. Use a Lineweaver-Burk plot to analyze the same data. a. Determine V and Ka from the Lineweaver-Burk BONUS: If the total enzyme concentration was I nmol/L, what is K?
Enzyme Kinetics Problem The initial rate for an enzyme-catalyzed reaction...
What is the rate of an enzyme catalyzed reaction if the Vmax is 100µmol S→P/min and...
What is the rate of an enzyme catalyzed reaction if the Vmax is 100µmol S→P/min and the Km is 7 mM and the substrate concentration is 11mM? Is the enzyme working at Vmax? What if the substrate concentration is raised to 25mM?
4. The following data were obtained for an enzyme-catalyzed reaction. Determine whether the enzyme is an allosteric enzyme. (5pts) Submit graph [S] (M X 104) v (umoles/liter/min) 1 1 2 3 4 5 6 7 2 4 7 15 35 55 75 80 83 85 85 8 9 10 11 12
3. The following data were obtained for an enzyme-catalyzed reaction. Determine whether the enzyme obeys hyperbolic or sigmoidal kinetics. [S] (M X 10) v (umoles/liter/min) 6.25 12.5 1.54 5.88 25 20 50 100 200 400 50 80 94.12 98.46 99.61 800
4. The following data were obtained for an enzyme-catalyzed reaction. Determine whether the enzyme obeys hyperbolic or sigmoidal kinetics. SUBMIT GRAPH [S] (MX 104) v (umoles/liter/min) V 6.25 12.5 25 50 100 200 400 800 1.54 5.88 20 50 80 94.12 98.46 99.611
3.33 3. Suppose that the following data are obtained for an enzyme-catalyzed reaction: [S(mm) V (mmol ml-Imin-1) 0.1 0.2 5.00 7.14 8.0 1.0 8.33 9.09 (a) From a double-reciprocal plot of the data, determine Km and Vmax. (b) Assuming that the enzyme present in the system had a concentration of 10-6 M, calculate its turnover number 0.8 2.0
20.8 2) Consider the following data for an enzyme-catalyzed hydrolysis reaction in the presence and absence of inhibitor I using a Michaelis-Menten plot, determine Kn for both inhibited and uninhibited reactions. What kind of inhibition is this? [Substrate)(M) vo (umol/min) Vos (moles/min) 6x106 4.2 1x105 29 5.8 2x105 6x109 13.6 1.8x10* 16.2
The reaction, A+B+C Products, was studied kinetically and the following data were obtained. d[A] 110M- 7.5 [A). [B] [C]. dt 0.010 0.005 0.010 5 0.010 0.005 0.015 0.010 0.015 0.010 45 0.020_0.005_0010 14.1 A. Determine the integrated rate law for the reaction. B. Determine the rate constant.
Enzyme Kinetics Problem The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate concentrations. Data are given below: 5 27 23 65 1. Estimate V and K from a Michaelis-Menten graph of V versus [S] 2. Use a Lineweaver-Burk plot to analyze the same data. a. Determine V and Ka from the Lineweaver-Burk BONUS: If the total enzyme concentration was I nmol/L, what is K?
Enzyme Kinetics Problem The initial rate for an enzyme-catalyzed reaction...
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