a) What is the role of the sensitivity of PFK-1 to the activity of the bifunctional...
a) What is the role of the sensitivity of PFK-1 to the activity of the bifunctional protein PFK-2/FBPase-2 in its production of F-2,6-phosphate
b) what are the consequences of this on the glycolytic and gluconeogenesis pathways?
Homework Answers
a) Fructose 2,6 bisphosphate is produced by the activity of the enzyme PFK2 and is broken down by FBPase-2, these enzymatic activities are catalyzed by a single bifunctional protein. Fructose 2,6 bisphosphate is an allosteric activator of PFK-1 when PFK2/FBPase-2 is phosphorylated by cAMP-dependent protein kinase FBPase active and PFK-2 is inactive so Fructose 2,6 bisphosphate is broken down and PFK-1 is inhibited. and when PFK2/FBPase-2 is dephosphorylated PFK-2 is active so produces more Fructose 2,6 bisphosphate which activates PFk-1.
b) when PFK-1 is active it favours glycolysis that is when PFK2/FBPase-2 is dephosphorylated glycolysis is favoured and when PFK2/FBPase-2 is phosphorylated FBPase-2 is active so it favours gluconeogenesis.
Add Answer to:
a) What is the role of the sensitivity of PFK-1 to the activity
of the bifunctional...
MATCH THE FOLLOWING a. PFK-1 b. FBpase-1 c. PFK-2 d. FBpase-2 e.cAMP- dependent protein kinase (PKA)...
MATCH THE FOLLOWING a. PFK-1 b. FBpase-1 c. PFK-2 d. FBpase-2 e.cAMP- dependent protein kinase (PKA) WITH 1. makes F-2,6 -BP(fructose-2,6-biphosphate) 2. Activated by F-2,6-BP(fructose-2,6-biphosphate) 3. Activates the enzyme that breaks down F-2,6-BP 4. Inhibited by F-2,6-BP 5. Breaks down F-2,6-BP
MATCH THE FOLLOWING a. PFK-1 b. FBpase-1 c. PFK-2 d. FBpase-2 e.cAMP- dependent protein kinase (PKA) WITH 1. makes F-2,6 -BP(fructose-2,6-biphosphate) 2. Activated by F-2,6-BP(fructose-2,6-biphosphate...
MATCH THE FOLLOWING a. PFK-1 b. FBpase-1 c. PFK-2 d. FBpase-2 e.cAMP- dependent protein kinase (PKA) WITH 1. makes F-2,6 -BP(fructose-2,6-biphosphate) 2. Activated by F-2,6-BP(fructose-2,6-biphosphate) 3. Activates the enzyme that breaks down F-2,6-BP 4. Inhibited by F-2,6-BP 5. Breaks down F-2,6-BP
Les one phonphate A) (3 pts) What is the role of fructose 6-phosphate in the PFK...
Les one phonphate A) (3 pts) What is the role of fructose 6-phosphate in the PFK catalyzed reaction as seen in graph Arch (A) Substrate; (B) Allosteric inhibitor; (C) Allosteric activator; (D) None of the above B) (3 pts) What is the role of fructose 2.6-bisphosphate (F-2,6-BP) in the PFK catalyzed reaction as seen Choose between: (A) Substrate; (B) Allosteric inhibitor; (C) Allosteric activator; (D) None of the above C) (4 pts) What are the 2 roles of ATP in...
PART A OPTIONS FOR BLANKS: 1- inhibits, does not affect, stimulates 2- fructose-1,6-biphosphatase-1, pyruvate kinase, phosphofructokinase-1...
PART A OPTIONS FOR BLANKS:
1- inhibits, does not affect, stimulates
2- fructose-1,6-biphosphatase-1, pyruvate kinase,
phosphofructokinase-1
3- phosphofructokinase-1, fructose-1,6-biphosphatase-1,
phosphoenolpyruvate carboxylate
PART B OPTIONS FOR BLANK:
Activity Curve A, Neither, Activity Curve B
Part 1 (3 points) See Hint High levels of fructose-2,6-BP reciprocally regulate glycolysis and gluconeogenesis. Fructose-2,6-BP flux through the glycolytic pathway by increasing the activity of Flux through the gluconeogenic pathway is inhibited by fructose-2,6- BP, which decreases the activity of Part 2 (1 point) See Hint...
Training Question 3. Shown below is the activity profile for the enzyme phosphofructokinase-1 (PFK-1) which catalyzes...
Training Question 3. Shown below is the activity profile for the enzyme phosphofructokinase-1 (PFK-1) which catalyzes the reaction: Fructose 6-phosphate + ATP → fructose 1,6-biphosphate + ADP. Low (ATPI PFK-1 activity High (ATP) [Fructose 6-phosphatel Describe the different patterns of regulation for different ATP levels. Explain the why such regulation is appropriate for this enzyme, given its role in metabolism.
During clinical rounds, you encounter patients who exhibit defects that affect gluconeogenesis. Predict the effect that...
During clinical rounds, you encounter patients who exhibit defects that affect gluconeogenesis. Predict the effect that each of the following conditions/defects would have on gluconeogenesis. Explain your prediction and indicate what life style change the patient might make to alleviate the symptoms. a) Patient 1 exhibits a deficiency of the liver lactate dehydrogenase activity. b) Patient 2 exhibits defective liver glucagon receptors which cannot bind glucagon. c) Patient 3 exhibits a constitutively phosphorylated liver phosphofructokinase-2 to lock the bifunctional enzyme...
This discussion focuses on the regulation of glycolysis and gluconeogenesis by phosphofructokinase-2 and will help you...
This discussion focuses on the regulation of glycolysis and
gluconeogenesis by phosphofructokinase-2 and will help you apply
your understanding of these pathways and their regulation to
adaptations in cancerous cells.
You have successfully completed your internship rotation
with the antibiotic group at
MethylTranspharmiX and have moved into
their Cancer Therapeutics division.
In many cancers, cells use aerobic glycolysis rather than
oxidative phosphorylation as their main energy source. This is
known as the Warburg effect, and was first described by Otto...
29. The activity of the enzyme phosphofructokinase is a) stimulated by low energy charge b) inhibited...
29. The activity of the enzyme phosphofructokinase is a) stimulated by low energy charge b) inhibited by fructose 2,6-bisphosphate c stimulated by fructose 1.6-bisphosphate d) all of the above 30. NADH is produced at a) aldolase catalyzed reaction b) Glyceraldehyde-3-phosphate dehydrogenase catalyzed reaction c) pyruvate kinase catalyzed reaction d) hexokinase catalyzed reaction 31. The transfer of 3-phosphoglycerate to 2-phosphoglycerate is catalyzed by which enzyme? a) PGM b)PK c)G2PDH d)TPI e)PFK 32. The enzyme aldolase catalyzes which reaction in Glycolysis? a)...
1.) Which of the following is the substrate or product of the reactions that comprise the...
1.) Which of the following is the substrate or product of the reactions that comprise the oxidative phase of the pentose phosphate pathway? A) glyceraldehyde-3-phosphate B) Ribulose-5-phosphate C) Seduheptulose - 7 - phosphate D) Ribose-5-phosphate 2) The regulated reactions of gluconeogenesis have/are A) Activated by molecules indicating a low energy charge in the cell B) reversible reactions C) those thare counter to regulated reactions in glycolysis D)Large positive change in G values 3) The biosynthesis of triacylglycerols in animals uses...
17. What is the net yield of the phos 1. Which of the follouing is not...
17. What is the net yield of the phos 1. Which of the follouing is not absorbed a. 32 ATP through the villi of the small intestine a monosaccharides b. farty acids b. 2ATP c IATP d. 3 ATP vitamins d. protein 18. What is the primary limiting factor of phosphagen system? a activation of CK b. depletion of creatine phosphate c. depletion of glycogen d. lack ofoxygen 12. How does glucose move across the epithelial cells into circulation? a...
Les one phonphate A) (3 pts) What is the role of fructose 6-phosphate in the PFK catalyzed reaction as seen in graph Arch (A) Substrate; (B) Allosteric inhibitor; (C) Allosteric activator; (D) None of the above B) (3 pts) What is the role of fructose 2.6-bisphosphate (F-2,6-BP) in the PFK catalyzed reaction as seen Choose between: (A) Substrate; (B) Allosteric inhibitor; (C) Allosteric activator; (D) None of the above C) (4 pts) What are the 2 roles of ATP in...
PART A OPTIONS FOR BLANKS:
1- inhibits, does not affect, stimulates
2- fructose-1,6-biphosphatase-1, pyruvate kinase,
phosphofructokinase-1
3- phosphofructokinase-1, fructose-1,6-biphosphatase-1,
phosphoenolpyruvate carboxylate
PART B OPTIONS FOR BLANK:
Activity Curve A, Neither, Activity Curve B
Part 1 (3 points) See Hint High levels of fructose-2,6-BP reciprocally regulate glycolysis and gluconeogenesis. Fructose-2,6-BP flux through the glycolytic pathway by increasing the activity of Flux through the gluconeogenic pathway is inhibited by fructose-2,6- BP, which decreases the activity of Part 2 (1 point) See Hint...
Training Question 3. Shown below is the activity profile for the enzyme phosphofructokinase-1 (PFK-1) which catalyzes the reaction: Fructose 6-phosphate + ATP → fructose 1,6-biphosphate + ADP. Low (ATPI PFK-1 activity High (ATP) [Fructose 6-phosphatel Describe the different patterns of regulation for different ATP levels. Explain the why such regulation is appropriate for this enzyme, given its role in metabolism.
This discussion focuses on the regulation of glycolysis and
gluconeogenesis by phosphofructokinase-2 and will help you apply
your understanding of these pathways and their regulation to
adaptations in cancerous cells.
You have successfully completed your internship rotation
with the antibiotic group at
MethylTranspharmiX and have moved into
their Cancer Therapeutics division.
In many cancers, cells use aerobic glycolysis rather than
oxidative phosphorylation as their main energy source. This is
known as the Warburg effect, and was first described by Otto...
29. The activity of the enzyme phosphofructokinase is a) stimulated by low energy charge b) inhibited by fructose 2,6-bisphosphate c stimulated by fructose 1.6-bisphosphate d) all of the above 30. NADH is produced at a) aldolase catalyzed reaction b) Glyceraldehyde-3-phosphate dehydrogenase catalyzed reaction c) pyruvate kinase catalyzed reaction d) hexokinase catalyzed reaction 31. The transfer of 3-phosphoglycerate to 2-phosphoglycerate is catalyzed by which enzyme? a) PGM b)PK c)G2PDH d)TPI e)PFK 32. The enzyme aldolase catalyzes which reaction in Glycolysis? a)...
17. What is the net yield of the phos 1. Which of the follouing is not absorbed a. 32 ATP through the villi of the small intestine a monosaccharides b. farty acids b. 2ATP c IATP d. 3 ATP vitamins d. protein 18. What is the primary limiting factor of phosphagen system? a activation of CK b. depletion of creatine phosphate c. depletion of glycogen d. lack ofoxygen 12. How does glucose move across the epithelial cells into circulation? a...
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