Explain the major advantages of “Confocal Microscopy” against standard fluorescence microscopy?
Explain the major advantages of “Confocal Microscopy” against standard fluorescence microscopy?
a) Fluorescent proteins such as green fluorescent protein (GFP) are commonly used in microscopy experiments. Describing the process of fluorescence, what is GFP and what is the main benefit of using fluorescent proteins over other fluorophores? b) Describe how fluorescent proteins can be used to (i) study a protein’s gene expression and (ii) localise proteins of interest within cells? c) The resolution of a standard confocal microscope is approximately 200nm. Describe an experiment that can be used to show whether...
2)Which of the A)peptidoglycan B)cellulose C)glycogen D)chitin 3 Which of the following is true of fluorescence microscopy? A) Fluorescence microscopy is best at viewing rounded, thicker specimens B) Fluorescent light is emitted throughout the specimen due to fluorescent dyes conjugated to antibodies. C) Fluorescence microscopy is used to view dead specimens only. D) Fluorescence microscopy presents images in three dimensions. E) Fluorescence microscopy is able to overcome problems encountered with using confocal scanning microscopy. 4)While synthesizing a new green pigment,...
To localize proteins and other types of molecules in cells: A. Fluorescence microscopy is often used. B. Labelled antibodies can be used to localize a type of protein. C. Labelled synthetic molecules that recognize a specific type of biomolecule (e.g. DNA) can be used. D. All of the above (ABC). E. None of the above
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron microscopy (EM]) could be used to observe each structure or phenomenon. Put "no" in the box if the technique could not be used. If light microscopy can be used, name one technique (bright-field, phase-contrast, fluorescence, etc.) that you think would be effective. You will find some useful information in Appendix 1 of this manual and Chapter 18 of your textbook. Structure or phenomenon Could...
Explain the advantages and disadvantages of the four major inventory costing methods.
1) GFP tagging is a commonly used method to label cells for visualization by fluorescence microscopy. Which of the following statement is most correct? a) The GFP tag can be visualized only in fixed cells b) The GFP tag can only be placed on the N-terminus of a protein c) The GFP tag can attach to the phospholipids of any membrane d) The GFP tag allows for visualizing the dynamic behavior of a protein by live imaging e) The GFP...
What are the three types of firms? Explain the major advantages and disadvantages of each. The three types of firms are O A. non-profit, non-governmental, and governmental O B. proprietorship, non-profit,, and corporation O C. governmental, partnership, and corporation O D. proprietorship, partnership, and corporation
Question 13 10 pts In a fluorescence spectrometer standard aqueous solutions of the fluorescent dye fluorescein are examined and yield the following fluorescence intensities: Concentration 0.00 2.00 4.00 6.00 8.00 10.00 12.00 [pg/mL] Fluorescence 5.00 9.0012.60 17.30 21.00 24.71 Intensity What is the 95% confidence interval for the expected y-intercept? Given, the standard deviation of the regression line is 0.187. 1.52 0.35 0-1.52 ± 0.074 1.93 0.74 1.53 +0.64
Question 13 10 pts In a fluorescence spectrometer standard aqueous solutions...
(microbiology) define/explain the major types of symbioses in which microorganisms engage. give exakples that explaijnrhe advantages and disadvantages for the symbiotic partners
(15 pts.) Infrared microscopy is valuable technique for characterizing materials. A. Explain the spatial resolution of infrared microscopy is significantly worse than the spatial resolution of Raman microscopy although they both are vibrational spectroscopy techniques. B. Why is a CCD not an appropriate detector for infrared imaging, but it is a useful detector for Raman imaging? 3.