An analyst has to decide which of 3 chromatography columns would be the best to buy based on the column efficiency in terms of the height equivalent of the theoretical plate.
Column 1: L=2.04m, TA= 9.13s, W1/2= 8.44s
Column 2: L=3.00m and H = 0.01 m
Column 3: L=5.00m and H = 0.03 m
Calculate the height of the theoretical plate (m) for column 1
An analyst has to decide which of 3 chromatography columns would be the best to buy...
Chromatography Basics Multiple Choice: Answer the following multiple choice questions about Chromatography Basics (SHOW WORKING): 1) In a given chromatographic column, A) different solutes behave as if there are a different number of theoretical plates for each. B) the number of theoretical plates is the same for all solutes. C) efficiency is improved by increasing plate height. 2) What useful information can be found from a Van Deemter plot? A) The selectivity factor B) Optimum mobile phase flow rate C) Optimum...
The plane frame in fig. 1 has columns of length h pinned at the base. The beam is welded to the columns and is of length 2l. Equal loads P are applied at the mid-span of both the beam and the right column. Find the collapse load PC in terms of MP , where MP is the plastic moment of the columns and beam. l is 3 m and h is 5m. The plane frame in fig. 1 has...
questions: 2,3, 4 and 5 please 2. How would the graph of temperature versus volume distilled appear for a perfect separation of methanol (bp 65.4°C) and toluene (bp 110°C) using an efficient frac- tionating column? 3. The efficiency of a fractional distillation is given as the number of cycles or theo- retical plates. Traversing through steps A - D in Figure 1H-1 is equivalent to hav- ing completed two vaporization-condensation cycles or two theoretical plates. Predict the temperature of the...
need help with number 2 HW #3 for Chem 318 Due: April 29, 2019 1. Solute A has a K 2.5 for an extraction between water (phase 1) and chloroform (phase 2). If 100 mL of a 0.01 fraction will be extracted? For the same solute and, what fraction will be extracted if 5 extractions wi 80 mL chloroform each are used (instead of one 400 ml extraction)? M solution containing solute A in water is extracted one time with...
what is the prediction of the unknown amino acids mixture based on the strip from thin layer chromatography sheet? The unknown mixtures can be ala plus asp, ala plus asp plus lys, lys plus asp or ala plus lys. anion exchange resin was used Tubes 2,3, 6 and 7 had a spot appear that was light pink We were unable to transcribe this imageWe were unable to transcribe this imageH-) to the column followed by a sufficient amount of the...
M3. EXTRACTION (5 p) 1. Fill in the blanks as shown below: is the distance of displacement of the liquids in the column due to the movement of the reciprocating piston of the pump during a cycle. b) The highest mass transfer performance will be achieved in regime. c) In extraction the separation of compounds is based on the differences in whereas in distillation it is based on the differences in volatilities. d) In an extraction column, the transfer rate...
1. Select an appropriate type of column for separating these steroids and give the rationale for your selection. HO HO ОН OH Prednisolone Prednisone 2. You are using a C18 Reverse Phase column and the parameters shown below to separate Acetaminophen, Caffeine, and Benzoic Acid. You observe three peaks in your chromatogram (Huzzah!). Which corresponds to which compound? Explain the rationale behind your assignment. OH C18 REVERSED PHASE COLUMN Acetaminophen HC N H, O Caffeine Column: Legacy L1 Size: 4.6...
Matching... Write the letter beside that which best describes the following. 1. _____ b-mercaptoethanol a. recognizes LVPRGS and cleaves the R-G peptide bond 2. _____ chymotrypsin b. purification technique used to separate proteins on the basis of charge 3. _____ trypsin c. utilizes DNA manipulation to co-express functional protein attachment 4. _____ cyanogen bromide d. purification technique used to separate proteins on the basis of size 5. _____ sodium dodecylsulfate e. is an example of an amphipathic molecule used...
ID: A Exam 3 Multiple Choice Identify the choice thar best completes the worawer the question 1. Le Chatelier's principle states that a. Only exothermic reactions can reach chemical equilibrium b. If a chemical equilibrium is disturbed, the system will try and reestablish equilibrium c. If a chemical equilibrium is disturbed, the temperature of the system will always decrense d. Only reactions in which a catalyst is present can reach chemical equilibrium 2. Which of following statements concerning theoretical yield,...
For the table below, design an ion exchange system for the removal of calcium. The flow rate to be treated is 5.45 ML/d. The system should be sized so that the minimum time between regenerations is 72 h. The column diameter should be 3 m and there should be at least 2 columns. Pilot testing indicates optimal regeneration efficiency corresponded to using 10% NaCl solution (specific gravity = 1.07) at a salt usage rate of 310 kg NaCl per m3...