Question

Matching... Write the letter beside that which best describes the following.  

                 

1. _____ b-mercaptoethanol	a. recognizes LVPRGS and cleaves the R-G peptide bond
2. _____ chymotrypsin	b. purification technique used to separate proteins on the basis of charge
3. _____ trypsin	c. utilizes DNA manipulation to co-express functional protein attachment
4. _____ cyanogen bromide	d. purification technique used to separate proteins on the basis of size
5. _____ sodium dodecylsulfate	e. is an example of an amphipathic molecule used to give proteins a uniform charge
6. _____imidazole	f. used to determine the N-terminus of a peptide
7. _____cation exchange	g. used to determine the C-terminus of a peptide
8. _____ affinity chromatography	h. cleaves after Phe, Trp, and Tyr residues
9. _____ sodium chloride	i. chemical used to elute from cation exchange column
10. _____ SDS-PAGE	j. cleaves after methionine residues
11. _____ dialysis 12. _____ thrombin 13. _____ Edman reagent 14. _____ carboxypeptidase	k. cleaves after arginine residues l. used to purify using centrifugal force m. used for exchanging buffers and/or desalting n. cleaves after aspartate and glutamate residues o. cleaves after lysine and arginine residues p. used to assess composition of samples to ascertain purity q. used to break disulfide bonds r. chemical used to elute proteins from a metal chelation column s. used to denature proteins by disrupting H-bonds